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| 响应面法优化产邻苯二酚培养基 | |
| 引用本文： | 张敏,何熙璞,李俊芳,王邕,李然,武波.响应面法优化产邻苯二酚培养基[J].广西农业生物科学,2008,27(2). |
| 作者姓名： | 张敏 何熙璞 李俊芳 王邕 李然 武波 |
| 作者单位： | 1. 广西大学生命科学与技术学院,广西,南宁,530005 2. 广西大学化学化工学院,广西,南宁,530004 3. 广西大学生命科学与技术学院,广西,南宁,530005;微生物及植物遗传工程教育部重点实验室,广西,南宁,530005;广西亚热带生物资源保护利用重点实验室,广西,南宁,530005 |
| 摘 要： | 采用快速有效的数学统计方法,对BGZ-06菌株利用苯甲酸钠产邻苯二酚的培养基进行了优化。首先通过Plackett-Burman的二水平因素设计筛选出较重要的因素,然后再利用响应面分析法中的杂合设计进行优化。优化后的培养基使邻苯二酚的产量提高了20.66%,获得的预测产量与实际产量之间具有较好的拟合度。 |
| 关 键 词： | 优化 邻苯二酚 Plackett-Burman设计 杂合设计 |
Optimization of catechol production medium by response surface analysis |
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| ZHANG Min,HE Xi-pu,LI Jun-fang,WANG Yong,LI Ran,WU Bo.Optimization of catechol production medium by response surface analysis[J].Journal of Guangxi Agricultural and Biological Science,2008,27(2). | |
| Authors: | ZHANG Min HE Xi-pu LI Jun-fang WANG Yong LI Ran WU Bo |
| Institution: | ZHANG Min1,HE Xi-pu2,LI Jun-fang1,WANG Yong1,LI Ran1,WU Bo1,3,4 |
| Abstract: | Efficient mathematical statistics were applied to optimize the medium for the strain BGZ-06 to produce catechol from benzoate.Two-level factorial design of Plackett-Burman was used to select important components for the medium,which were then optimized by the hybrid design of response surface analysis.The catechol production by strain BGZ-06 grown in the optimized medium increased 20.66% compared to that grown in the original medium.Experimental data revealed that the virtual catechol yield of BGZ-06 was consistent with the predicted yield by the response surface analysis. |
| Keywords: | optimization catechol Plackett-Burman design hybrid design |
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