| 木霉菌素产生菌的诱变育种 |
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| 引用本文： | 石一珺,申屠旭萍,俞晓平.木霉菌素产生菌的诱变育种[J].安徽农业科学,2008,36(29). |
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| 作者姓名： | 石一珺 申屠旭萍 俞晓平 |
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| 作者单位： | 杭州师范大学生命与环境科学学院,浙江杭州,310034;中国计量学院生命科学学院,浙江杭州,310018;浙江省农业科学院植物保护与微生物研究所,浙江杭州,310021 |
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| 基金项目： | 浙江省自然科学基金
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浙江省重大招标项目
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| 摘 要： | 目的]筛选高产木霉菌素菌株,提高木霉菌素产量。方法]以从枸骨中分离到的产木霉菌素的内生真菌———哈茨木霉为出发菌株,进行紫外线二次复合诱变处理,将筛选出的突变菌株连续转接5代,测定其遗传稳定性。结果]随着照射时间的延长,哈茨木霉的致死率增大,选取致死率为88.1%的紫外线照射45 s作为最适处理剂量进行诱变育种。经过2次诱变的菌株的产孢时间提前,长出的菌落更为致密。经过初筛和复筛,最终获得1株高产突变株UV-5-3,其产抗生素的水平最高,为164.75μg/m l,比初次诱变筛选获得的突变株UV-3-1提高了56.77%,是出发菌株的2.3倍。传代试验表明,突变株UV-5-3的高产性能遗传特性稳定。结论]利用紫外线二次复合诱变处理哈茨木霉可以获得高产木霉菌素菌株。
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| 关 键 词： | 木霉菌素 哈茨木霉 紫外诱变 筛选 |
Study on Mutation Breeding of Trichodermin Producing Strain |
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| Abstract: | Objective] The aim was to screen the high yield trichodermin strain and increase the yield of trichodermin.Method] With Trichoderma harzianum,an endophytic fungi producing trichodermin separated from Llex cornuta,as the original strain for the treatment of ultraviolet radiation compound mutation for two times,the 5 generations of the screened mutation strains were transferred continuously and its genetic stability was detected.Result] The lethality rate of T.harzianum was increased with the prolonging of the irradiation time.The ultraviolet irradiation of 45 s with the lethality rate of 88.1% was selected as the optimum mutation dose for mutation breeding.The sporulation time of the strain through twice mutation was advanced and the growing strain was dense.A high yield mutation strain UV-5-3 was obtained at last through the primary screening and the secondary screening and its antibiotic level was the highest,being 164.75 μg/ml,which was 56.77% higher than that of UV-3-1 mutation strain obtained through the primary screening,and the 2.3 times of the original strain.The passage experiment showed that the high yield preference of the mutation strain UV-5-3 had stable genetic characteristic.Conclusion] The high yield trichodermin strain could be obtained by treating T.harzianum through ultraviolet radiation compound mutation treatment for two times. |
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| Keywords: | Trichodermin Trichoderma harzianum Ultraviolet radiation mutation Screen |
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