| 铬胁迫对紫花苜蓿种子发芽及幼苗生理生化特性的影响 |
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| 引用本文： | 尹国丽,寇江涛,师尚礼,陈本建,周万海.铬胁迫对紫花苜蓿种子发芽及幼苗生理生化特性的影响[J].青海草业,2013,22(2):2-8. |
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| 作者姓名： | 尹国丽 寇江涛 师尚礼 陈本建 周万海 |
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| 作者单位： | 甘肃农业大学草业学院,甘肃兰州730070;草业生态系统教育部重点实验室,甘肃省草业工程实验室,中-美草地畜牧业可持续研究中心,甘肃兰州730070 |
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| 基金项目： | 国家现代牧草产业技术体系建设专项，全国种质资源保种，农业部行业专项"牧区优质高效饲草生产利用技术研究与示范" |
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| 摘 要： | 以"甘农3号"紫花苜蓿(Medicago sativa L.cv.Gannong No.3)品种为试验材料,研究不同浓度Cr(0、0.5、1.0、2.5、5.0、10.0 mmol·L-1CrCl3)胁迫对紫花苜蓿种子萌发及幼苗生理生化特性的影响。结果表明:Cr胁迫显著抑制了紫花苜蓿种子的萌发及幼苗的生长,种子发芽势、发芽率及幼苗的胚芽长、胚根长和干重均显著降低(p<0.05),且对发芽势的抑制程度大于发芽率,对胚根生长的抑制程度大于胚芽。在低浓度Cr胁迫(0.5和1 mmol·L-1)下,苜蓿幼苗可溶性蛋白质含量、可溶性糖含量、蛋白质水解酶活性及超氧化物歧化酶(SOD)活性与CK差异不显著,过氧化氢酶(CAT)活性下降,抗坏血酸过氧化物酶(APX)、愈创木酚过氧化物酶(GPX)活性升高,当Cr胁迫浓度达到2.5 mmol·L-1时,可溶性蛋白质含量、可溶性糖含量下降,蛋白质水解酶、SOD、CAT活性均显著受到抑制,APX、GPX活性也显著下降。随着Cr胁迫浓度的增加,超氧阴离子自由基产生速率、H2O2及丙二醛(MDA)含量均显著增加。说明低浓度Cr胁迫下,苜蓿幼苗抗氧化系统清除活性氧的能力上升,高浓度Cr胁迫下,苜蓿幼苗抗氧化系统活性下降,膜脂过氧化程度加剧,这是苜蓿幼苗生物量下降的主要原因。可见,紫花苜蓿对重金属Cr胁迫产生的适应性是有一定条件的。
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| 关 键 词： | 铬胁迫 紫花苜蓿 种子萌发 幼苗生长 抗氧化酶 活性氧 |
THE EFFECT OF CR STRESS ON GERMINATION AND PHYSIOLOGICAL-BIOCHEMICAL CHARACTERISTICS OF ALFALFA SEEDLINGS |
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| Institution: | YIN Guo-li;College of Grassland Science,Gansu Agricultural University; |
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| Abstract: | Medicago sativa L. cv. Gannong No. 3 was used to study the different for concentrattons u, u. 3, , 2.5, 5, 10 mmol ·L-1 CrC13 ）on seed germination and seedling physiological -biochemical characteristics. The results indicating that Cr inhibited seed germination and radicle and plumule lengths, seed germination energy and dry seedling growth significantly, under which the weight all decreased significantly （ p 〈 0.05 ） , and the inhibition effect of Cr on germination energy and radicle growth were found significantly higher than ger- mination rate and plumule growth, respectively. Under low Cr stress concentrations （0.5 and 1 mmol L-1 ） , no significant difference was found from CK in soluble protein content, soluble sugar content, proteolytic enzyme activity and SOD activity, the CAT activity decreased, while APX activity increased. Under 2.5 mmol ~ I.- ~ Cr concentration, soluble protein and soluble sugar content decreased, and the activities of proteolytic enzyme, SOD and CAT were inhibited, APX and GPX activities decreased significantly, indicating that low Cr concen- trations promoted the capability of antioxidant system in eliminating reactive oxygen, while under high Cr con- centrations, antioxidant system activity in seedlings decreased, and the lipid peroxidation accelerated, which were the main reason explaining the decrease of seedling biomass. It is visible that the adaptability generation of alfalfa seedlings to Cr needs certain conditions. |
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| Keywords: | Cr stress Alfalfa Seed germination Seedling growth Antioxidant enzyme Reactive oxygen |
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