| 蝴蝶兰花葶的离体培养 |
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| 引用本文： | 陈之林,叶秀粦,梁承邺.蝴蝶兰花葶的离体培养[J].园艺学报,2003,30(2):242-244. |
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| 作者姓名： | 陈之林 叶秀粦 梁承邺 |
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| 作者单位： | (中国科学院华南植物研究所，广州510650) |
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| 基金项目： | 中国科学院院长基金资助项目 (76 7)，广东省科技计划资助项目 (C2 0 30 4) |
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| 摘 要： | 蝴蝶兰花梗腋芽培养在含VW无机盐+肌醇100 mg·L-1+VB110 mg·L-1+VB6 mg·L-1+烟酸1 mg·L-1+蔗糖20g·L-1+BA 5.0 mg·L-1的培养基上22°C培养，可分化出花葶。花葶切片在含Ms无机盐+肌醇100mg·L-1 +VB110 mg·L-1+VB61 mg·L-1+烟酸1 mg·L-1+蔗糖15 g·L-1+BA 5.0 mg·L-1+NAA0．5 mg·L-1 的培养基上诱导分化不定芽。不定芽在同一培养基上继代增殖，在附加香蕉汁100 g·L 的Hyponex 1号生根培养基上壮苗生根。
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| 关 键 词： | 蝴蝶兰 花葶 不定芽 离体培养 |
| 文章编号： | 0513-353X(2003)02-0242-03 |
| 收稿时间： | 2002-7-3 |
| 修稿时间： | 2002年7月3日 |
In Vitro Culture of the Inflorescence of Phalaenopsis |
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| Chen Zhilin,Ye Xiulin,and Liang Chengye.In Vitro Culture of the Inflorescence of Phalaenopsis[J].Acta Horticulturae Sinica,2003,30(2):242-244. |
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| Authors: | Chen Zhilin Ye Xiulin and Liang Chengye |
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| Institution: | (South China Institute of Botany，Academia Sinica，Guangzhou 510650，China) |
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| Abstract: | Inflorescences were induced by culturing flower-stalk buds of Phalaenopsis on V&;W medium containing vitamin component of B5 medium and 5.0 mg·L-1 benzylaminopurine(BA) under 22°C．Adventitious shoot were induced from the intemodal segments of inflorescence by using the NS medium containing vitamin of B5 medium 0.5 mg·L-1BA and 0. mg·L-1 NAA．The low sucrose concentration benefits the shoot's diferentiation and growth． Shoot were propagated on the same medium and developed into plantlet on Hyponex 1 nlediuln containing 100 g·L-1 banana juice and 2 g·L-1 active charcoal． |
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| Keywords: | Phalaenopsis Inflorescences Adventitious shoot In vitro culture |
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