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茄子青枯病抗性基因的遗传分析及其AFLP标记
引用本文:李猛,王永清,田时炳,罗章勇,王小佳.茄子青枯病抗性基因的遗传分析及其AFLP标记[J].园艺学报,2006,33(4):869-872.
作者姓名:李猛  王永清  田时炳  罗章勇  王小佳
作者单位:( 西南大学园艺园林学院, 重庆400716; 重庆市农业科学研究所, 重庆400055)
基金项目:贵州省‘十一五’蔬菜攻关项目(黔科合NY字2006-3001)
摘    要: 以茄子高感青枯病品种‘三月茄’与高抗品种‘S69’的F1、F2为材料, 对青枯病抗性遗传进行了分析, 同时开展了与抗性基因连锁的AFLP标记鉴定。结果表明: ‘S69’对青枯病的抗病性属于不完全隐性遗传, 感病性属于不完全显性; 抗性由1~2对基因控制。对86个F2单株AFLP分析, 鉴定出1个与‘S69’抗性基因连锁的AFLP标记39A980 (该引物组合为E-ACC /M-CTG) , 该标记与抗性基因间的交换值为4.65% , 遗传距离为4.9 cM。

关 键 词:茄子  青枯病  抗性遗传  AFLP标记
文章编号:0513-353X(2006)04-0869-04
收稿时间:2006-01-26
修稿时间:2006-01-262006-06-20

Genetic Analysis of Resistance to Bacterial Wilt and Identification of an Associated AFLP Marker in Eggplant (Solanum melongena)
Li Meng,Wang Yongqing,Tian Shibing,Luo Zhangyong,Wang Xiaojia.Genetic Analysis of Resistance to Bacterial Wilt and Identification of an Associated AFLP Marker in Eggplant (Solanum melongena)[J].Acta Horticulturae Sinica,2006,33(4):869-872.
Authors:Li Meng  Wang Yongqing  Tian Shibing  Luo Zhangyong  Wang Xiaojia
Institution:(College of Horticulture and Landscape, Southwest University, Chongqing 400716, China; Chongqing Institute of Agricultural Sciences, Chongqing 400055, China)
Abstract:F1 and F2 populations, derived from the cross between bacterialwilt highly susceptible cultivar ‘Sanyueqie’and highly resistant Indonesian cultivar‘S69’, were used for investigation of the inheritance of resistance to bacteria wilt and for identification of associated AFLP markers in eggplant. The results showed that the resistance to bacterialwiltwas incompletely recessive, otherwise susceptibilitywas incomp letely dominant, which was controlled by 1 - 2 genes. By using a combination of the amp lified fragment length polymor2phism (AFLP) technique and bulked segregant analysis (BSA) , an AFLP marker, named 39A980 was identified with the p rimer combination E-ACC /M-CTG to be linked to the major resistant gene. The crossing-over value and the genetic distance between the resistant gene and‘39A980 ’were estimated as 4.65% and 4.9 cM respectively by analyzing a set of 86 F2 plants.
Keywords:Solanum melongena  Bacterial wilt  Inheritance of resistance  AFLP markers
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