| 乳酸粪肠球菌的分离鉴定与系统进化分析 |
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| 引用本文： | 杨杨 霞,陈 陆,常洪涛,刘红英,杨影影,符 峰,王川庆.乳酸粪肠球菌的分离鉴定与系统进化分析[J].中国农学通报,2009,25(10):1-5. |
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| 作者姓名： | 杨杨 霞 陈 陆 常洪涛 刘红英 杨影影 符 峰 王川庆 |
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| 作者单位： | 1. 河南农业大学牧医工程学院,郑州,450002
2. 河南粮油饲料产品质量监督检验站,郑州,450003 |
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| 摘 要： | 目的 分离鉴定一株疑似乳酸粪肠球菌；方法 通过对表型特性包括培养特性、形态学观察、生化试验等鉴定，同时结合16S rDNA系统进化分析；结果：该株革兰氏阳性菌能在10℃和45℃生长，接触酶阴性，发酵葡萄糖产酸不产气，可生长于6.5%NaCl和PH9.6的环境等，16S rDNA系统进化分析与粪肠球菌有最高同源性；结论 分离株为乳酸粪肠球菌。
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| 关 键 词： | 乳酸粪肠球菌 生化鉴定 16SrDNA 进化分析 |
| 收稿时间： | 2008-12-08 |
| 修稿时间： | 2009-02-08 |
Isolation,Identification and Phylogenetic Analysis of a Strain Enterococcus Faecalis |
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| Yang Xi,Chen Lu,Chang Hongtao,Liu Hongying,Yang Yingying,Fu Feng,Wang Chuanqing.Isolation,Identification and Phylogenetic Analysis of a Strain Enterococcus Faecalis[J].Chinese Agricultural Science Bulletin,2009,25(10):1-5. |
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| Authors: | Yang Xi Chen Lu Chang Hongtao Liu Hongying Yang Yingying Fu Feng Wang Chuanqing |
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| Institution: | Yang Xia, Chen Lu, Chang Hongtao, Liu Hongying, Yang Yingying, Fu Feng, Wang Chuanqing (1Engineering College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou 450002; 2The supervision and testing station for grain, oil and feed products in henan province, Zhengzhou 450003) |
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| Abstract: | Abstract: Objective Isolation and Identification of a doubtful enterococcus faecalis strain;Methods through Phenotypic Characteristics containing culture characteristics, Morphological observation, biochemistry experiment etc, combining with the phylogenetic analysis of 16S rDNA; Results The result indicated that this Gram-positive bacteria can grow at 10 ℃ and 45℃,6.5% sodium chloride or pH9.6 also.catalase- negative,can produce acid and no gas from glucose. though phylogenetic analysis of 16S rDNA,we can found that it has topmost homogeneity with E.faecalis;Conclusion These findings may indicated that the isolated strain should be E.faecalis. |
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| Keywords: | 16S rDNA |
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