| 烟草赤星病拮抗生防菌BS06-1的筛选 |
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| 引用本文： | 唐圣华,万秀清,郭兆奎,颜培强,李丽杰.烟草赤星病拮抗生防菌BS06-1的筛选[J].安徽农业科学,2008,36(35). |
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| 作者姓名： | 唐圣华 万秀清 郭兆奎 颜培强 李丽杰 |
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| 作者单位： | 1. 安徽中烟工业公司蚌埠卷烟厂,安徽蚌埠,233000
2. 牡丹江烟草科学研究所,黑龙江牡丹江,157011 |
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| 基金项目： | 黑龙江省烟草专卖局资助项目 |
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| 摘 要： | 目的]筛选对烟草赤星病具有明显拮抗作用的细菌。方法]应用平板对峙培养法对从烟草根际分离到的细菌进行烟草赤星病拮抗性鉴定,对其中对烟草赤星病菌拮抗性较高的一株细菌利用细菌16S rRNA通用鉴定引物进行PCR扩增、测序和田间抗病性试验。结果]共从烟草根际分离120份细菌,有6份对烟草赤星病病原菌具有一定拮抗作用,其中1份细菌拮抗作用明显,定名为BS06-1。将该细菌的16S rRNA测序结果登录NCBI网站进行Blastn序列比对,结果表明该菌株与枯草芽孢杆菌BSD-2同源性达到96%,与枯草芽孢杆菌BCRC 14718同源性达到95%,证明该菌属于枯草芽孢杆菌。田间试验证明该菌株BS06-1对烟草赤星病具有显著的抑菌效果。结论]BS06-1细菌对烟草赤星病具有明显拮抗作用。
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| 关 键 词： | 枯草芽孢杆菌BS06-1 烟草赤星病 生防 |
Screening of Antagonistic Biocontrol Bacteria BS06 1 to Tobacco Brown Spot |
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| Abstract: | Objective] The study aimed to screen the bacterium that had obvious antagonism to tobacco brown spot.Method] The method of confront culture on a plate was used to isolate the bacteria from tobacco rhizosphere for antagonism identification of tobacco brown spot.One isolated bacterium that had high antagonism to tobacco brown spot was made for PCR amplification and sequencing by general bacterium 16S rRNA identification primer and as well as for disease resistance test in field.Result] There were 120 bacteria that were isolated from tobacco rhizosphere,among them,6 bacteria had some antagonism to tobacco brown spot in which one BS06-1 had obvious antagonism to tobacco brown spot and was named as BS06-1.When the 16S rRNA sequencing result of the bacterium was logged on NCBI website for Blastn sequence comparison,it was found that the bacterium showed 96% homology with Bacillus subtilis strain BSD-2 and 95% homology with B.subtilis BCRC 14718,proving that this bacterium belonged to B.subtilis The field test showed that the strain BS06-1 had significant inhibition to tobacco brown spot.Conclusion] The bacterium BS06-1 showed an obvious antagonism to tobacco brown spot. |
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| Keywords: | Bacillus subtilis strain BS06-1 Tobacco brown spot Antagonism |
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