Assessment of the Hemocue-b for measuring hemoglobin in horse blood |
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| Authors: | H Chevalier LP Posner JW Ludders HN Erb RD Gleed |
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| Institution: | Cornell University, Ithaca, NY, USA |
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| Abstract: | Our purpose was to assess the accuracy and precision of a point of care hemoglobinometer (HemoCue‐B hemoglobin photometer) for measuring hemoglobin concentration in horse blood. Samples of jugular venous blood from 12 healthy adult horses were collected in EDTA. In order to test the device over a wide range of values, each sample was divided into nine aliquots, and autologous plasma was added or removed from the aliquots to produce blood with PCV values that approximated 5, 10, 20, 30, 40, 50, 60, 70, and 80%, respectively. The aliquots were rocked to ensure mixing of plasma and cells. Then hemoglobin by HemoCue‐B (HbHQ) and hemoglobin by the cyanmethemoglobin method (HbCY) were measured on each aliquot. The PCV of each aliquot was also measured and this value was used for subsequent analyses. To test repeatability, hemoglobin was measured twice by the HemoCue‐B on approximately 40% samples. Samples with HbHQ >25.4 g dL?1 required dilution prior to analysis. HbCY ranged from 1.6 to 33.4 g dL?1. After regression, HbCY = ?0.16 + 1.04 HbHQ (n = 101; r2 = 99.6%). By inspection of a modified Bland‐Altman plot, HbHQ values <16 g dL?1 closely approximated HbCY; however, at greater values, HbHQ underestimated HbCY by as much as 3.2 g dL?1. The difference between repeated measurements with the HemoCue‐B was 0.02 ± 0.16 g dL?1 (mean ± SD; n = 10) and nonsignificant. After regression, PCV = ?0.76 + 2.78 HbHQ (n = 101; r2 = 99.4%). We conclude that HemoCue‐B can be used to measure hemoglobin concentration in horse blood, and that it is accurate when hemoglobin is <16 g dL?1. PCV can be estimated by multiplying HbHQ by 2.8 and then subtracting 0.8. |
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