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| 球孢白僵菌MZ050724菌株对禾谷缢管蚜的毒力测定 | |
| 引用本文： | 孔琼,袁盛勇,张宏瑞,郭亚力,田学军,张薇.球孢白僵菌MZ050724菌株对禾谷缢管蚜的毒力测定[J].安徽农业科学,2008,36(29). |
| 作者姓名： | 孔琼 袁盛勇 张宏瑞 郭亚力 田学军 张薇 |
| 作者单位： | 1. 红河学院生物系,云南蒙自,661100 2. 红河学院农学系,云南蒙自,661100 3. 云南农业大学植物保护学院,云南昆明,650201 |
| 基金项目： | 红河学院博士、硕士科研启动项目 |
| 摘 要： | 目的]探讨球孢白僵菌的侵染致病力及其开发潜力,为禾谷缢管蚜的高毒力菌株的筛选提供理论依据。方法]采用PDA培养基培养球孢白僵菌(Beauveryia bassiana)MZ050724菌株分生孢子。测定了不同浓度的分生孢子液对禾谷缢管蚜的毒力,将其最佳防效的浓度分别与稀释10倍和100倍的36%阿希米、30%胜蚜、10%比其乐、10%赛比4种化学农药混配,测定其增效作用。结果]球孢白僵菌MZ050724菌株在4.2×107个/m l浓度下对禾谷缢管蚜的毒力效果较好,其累计校正死亡率为60.87%;浓度为4.2×107个/m l分生孢子液与4种农药混配,10%比其乐的稀释液增效高达4%,30%胜蚜稀释100倍液最低达到-25%,10%塞比稀释100倍液增效达6%,而与其他混用则表现为拮抗。结论]10%比其乐可湿性粉剂与球孢白僵菌MZ050724菌株混用可以达到互补效果。 |
| 关 键 词： | 球孢白僵菌 禾谷缢管蚜 毒力 化学农药 |
Determination on Virulence of Beauveryia bassiana MZ050724 on Rhopalosiphum padi L |
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| Abstract: | Objective] The disease infection of Beauveria bassiana and its development potential were discussed for a theoretical basis of the strain-screening with highly toxic to Rhopalosiphum padi aphid.Method] The PDA medium was used to culture Beauveria bassiana strain-MZ050724 spores.The toxicity effect of the different concentrations of spore solutions on Rhopalosiphum padi aphid was determined.The synergies of the combination of its best-effective concentration and four kinds of chemical pesticides(36% Eximi,30% Shengya,10% Biqile and 10% Sabi) the diluted solution by 10 times and 100 times was respectively measured.Results] The Beauveria bassiana strain-MZ050724 with the concentration of 4.2×107 spores/ml showed better toxicity performing to Rhopalosiphum padi aphid(the cumulative correction death rate: 60.87%).In the optimal concentration of Beauveria bassiana strain-MZ050724 mixed with four kinds of chemical pesticides respectively,the efficiency of the solution mixed with 10% Biqile could be 4% higher;the minimum from 30% Shengya diluted by 100 times reached-25%;10% Sabi diluted by 100 times could be 6% higher and there was antagonism while it mixed with other manifestations.Conclusion] The mixture of 10% Biqile and Beauveria bassiana strain-MZ050724 can achieve the complementary effect. |
| Keywords: | Beauveria bassiana Rhopalosiphum padi aphid Toxicity Chemical pesticide |
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