| 抗水牛伊氏锥虫变异表面糖蛋白抗原单克隆抗体的研制 |
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| 引用本文： | 曾晓飞,陈汉忠,韦英益,何木荣,李晓栩.抗水牛伊氏锥虫变异表面糖蛋白抗原单克隆抗体的研制[J].中国畜牧兽医,2009,36(10):72-74. |
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| 作者姓名： | 曾晓飞 陈汉忠 韦英益 何木荣 李晓栩 |
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| 作者单位： | （广西大学动物科技学院， 南宁 530005） |
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| 基金项目： | 国家自然科学基金资助项目，广西教育厅科研项目 |
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| 摘 要： | 用纯化的伊氏锥虫变异表面糖蛋白（variant surface glucoprotein, VSG）免疫BALB/c小鼠，取免疫小鼠脾细胞与SP2/0骨髓瘤细胞进行融合，经过3次克隆和间接ELISA方法筛选，获得3D7、5B9 2株稳定分泌单克隆抗体的杂交瘤细胞株，用间接ELISA方法检测杂交瘤细胞培养液上清效价和小鼠腹水效价，其中细胞培养上清效价分别为1∶6400和1∶12800，腹水效价分别为1∶105和1∶106。单抗的亚型鉴定结果表明，3D7、5B9分泌的抗体都为IgG1亚类κ链。
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| 关 键 词： | 变异表面糖蛋白 伊氏锥虫 单克隆抗体 |
Preparation of Monoclonal Antibody against Variant Surface Glucoprotein Antigen of Trypanosoma evansi |
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| ZENG Xiao-fei,CHEN Han-zhong,WEI Ying-yi,HE Mu-rong,LI Xiao-xu.Preparation of Monoclonal Antibody against Variant Surface Glucoprotein Antigen of Trypanosoma evansi[J].China Animal Husbandry & Veterinary Medicine,2009,36(10):72-74. |
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| Authors: | ZENG Xiao-fei CHEN Han-zhong WEI Ying-yi HE Mu-rong LI Xiao-xu |
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| Institution: | (College of Animal Science and Technology, Guangxi University, Nanning 530005, China) |
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| Abstract: | The BALB/c mice were immunized with variant surface glucoprotein of Trypanosoma evansi. The spleen lymph cells were fused with mouse SP2/0 myeloma cells, two hybridoma cell lines 3D7 and 5B9 secreting monoclonal antibodies against variant surface glucoprotein were determined by indirect ELISA. Their titers in cell culture supernatant were 1∶6400 and 1∶12800, and titers of ascites were 1∶105 and 1∶106, both 3D7 and 5B9 belongs to IgG1 with κ chain. |
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| Keywords: | variant surface glucoprotein Trypanosoma evansi monoclonal antibody |
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