| 麻疯树pepc基因正义、反义植物表达载体构建与功能初步分析 |
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| 引用本文： | 范正琪,卢孟柱,李纪元,田敏,李辛雷,吴开云,陈东亮,范妙华.麻疯树pepc基因正义、反义植物表达载体构建与功能初步分析[J].林业科学研究,2011,24(2):165-170. |
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| 作者姓名： | 范正琪 卢孟柱 李纪元 田敏 李辛雷 吴开云 陈东亮 范妙华 |
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| 作者单位： | 1. 中国林业科学研究院亚热带林业研究所,浙江,富阳,311400
2. 中国林业科学研究院林业研究所,北京,100091 |
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| 基金项目： | 浙江省重大科技专项重点项目"林源植物脂肪酸合成酶基因工程菌株的开发利用"(2006C12030) |
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| 摘 要： | 根据麻疯树pepc基因和植物表达载体的酶切位点特征,分别将pepc基因全长序列3 000 bp正向插入pCAMBIA2300,构建了正义表达载体pCAMBIA-Jcpepc,基因片段597 bp反向插入pBI121构建了反义表达载体pBIJcpepc.通过农杆菌介导,采用叶盘法转化烟草,通过对转基因植株的PCR和PCR...
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| 关 键 词： | 麻疯树 pepc基因 表达载体构建 转化 脂肪酸 |
| 收稿时间： | 2010/3/19 0:00:00 |
Constructing of Sense and Antisense pepc Expression Vector of Jatropha curcas and the Transformation |
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| FAN Zheng-qi,LU Meng-zhu,LI Ji-yuan,TIAN Min,LI Xin-lei,WU Kai-yun,CHEN Dong-liang and FAN Miao-hua.Constructing of Sense and Antisense pepc Expression Vector of Jatropha curcas and the Transformation[J].Forest Research,2011,24(2):165-170. |
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| Authors: | FAN Zheng-qi LU Meng-zhu LI Ji-yuan TIAN Min LI Xin-lei WU Kai-yun CHEN Dong-liang and FAN Miao-hua |
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| Institution: | Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China;;Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China;;Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China;;Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China;;Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China;;Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China;;Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China; |
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| Abstract: | Antisense pepc gene expression vector was constructed by the 597 bp sequence of pepc gene ligated to pBI121 in the antisense orientation. And sense expression vector was recombined by the full-length (3 000 bp) sequence inserted to pCAMBIA2300. Mediated by Agrobacterium tumefaciens, the sense and antisense genes were transferred into the tobacco using leaf-disk method. Results of PCR and PCR-Southern determination indicated two gene sequences were integrated into the tobacco genome in the sense and antisense orientation. The activity of PEPCase was determined. It was found that the fat content of transgenic tobacco with sense pepc was 44.3 percent lower than that of control, and that with antisense pepc was 26.3 percent higher than that of the control. |
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| Keywords: | Jatropha curcas pepc gene construction of the plant expression vector transformation fatty acid |
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