| 水稻广亲和基因在RFLP图谱上的初步定位 |
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| 引用本文： | 刘蔼民,李和标,张启发,姜晓红,师素云,杨官品.水稻广亲和基因在RFLP图谱上的初步定位[J].华中农业大学学报,1992,11(3). |
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| 作者姓名： | 刘蔼民 李和标 张启发 姜晓红 师素云 杨官品 |
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| 作者单位： | 江苏省农业科学院，江苏省农业科学院，华中农业大学，江苏省农业科学院，江苏省农业科学院，华中农业大学 南京 210014，南京 210014，武汉 430070，南京 210014，南京 210014，武汉 430070 |
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| 基金项目： | 华中农业大学作物遗传改良国家重点实验室开放基金，江苏省科委资助~~ |
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| 摘 要： | 以02428/Balilla//南京11三交F_1群体为材料,对利用染色体特异的RFLP标记进行水稻广亲和基因的定位作了初步尝试。结果表明:该群体中单株小穗可育率呈双峰分布,高结实株与低结实株之比符合1:1分离规律,说明水稻广亲和性受一对显性核基因控制;所用7个已定位于第6染色体的探针中,RG213和RG138在02428与另两个亲本之间表现多态性;广亲和基因与Est-2、RG213、RG138、C基因4个位点间均表现极显著连锁关系,交换值分别为5.9%,7.9%,9.7%和10.3%。广亲和基因在RFLP图上位置的初步确定为进行该基因的精细定位、克隆以及该基因的有效利用奠定了基础。
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| 关 键 词： | 水稻 广亲和基因 连锁分析 亚种间杂交 |
MAPPING A WIDE COMPATIBILITY GENE OF RICE IN RELATION TO RFLP MARKERS |
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| LIU Ai-min LI He-biao.MAPPING A WIDE COMPATIBILITY GENE OF RICE IN RELATION TO RFLP MARKERS[J].Journal of Huazhong Agricultural University,1992,11(3). |
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| Authors: | LIU Ai-min LI He-biao |
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| Abstract: | Wide compatibility (WC) gene in rice (Oryza sativa L.) is able to overcome the partial sterility which was frequently encountered in indica-japonica crosses. The objective f the present study was to map WC gene in relation to RFLP markers, using an F_1 population from a triparent cross. 02428/Balilla//Nanjing 11. Seven markers were selected from the RFLP map to represent a region on chromosome 6 which we had speculated to include the WC gene based on results from previous studies using morphological markers. Six of the seven probes were polymorphic among the three parents, but only two (RG138 and RG213) were found to be useful for mapping the WC gene with the triparent cross population. We also included two additional polymorphic loci. a chromogen gene locus (C) and an isozyme locus (Est2). as reference markers. It was observed that fully fertile and partly sterile plants segregated in a typical 1:1 ratio, confirming that the wide compatibility was governed by a major dominant gene. X~2 tests showed that all the four markers were linked to the WC gene. and the recombination frequencies between the WC gene and Est2. RG213. RG 138 and C were 5.9%, 7.9%. 9.7% and 10.3%, respectively. |
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| Keywords: | Oryza sativa triparent cross linkage analysis wide compatibility gene |
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