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| 香菇gpd启动子引导的云芝漆酶基因真核表达载体的构建 | |
| 引用本文： | 邓勋,宋瑞清,宋小双,闵凯,孙海珍.香菇gpd启动子引导的云芝漆酶基因真核表达载体的构建[J].东北林业大学学报,2009,37(9). |
| 作者姓名： | 邓勋 宋瑞清 宋小双 闵凯 孙海珍 |
| 作者单位： | 1. 黑龙江省森林保护研究所,哈尔滨,150040 2. 东北林业大学 3. 黑龙江省森林保护研究所 |
| 基金项目： | 黑龙江省自然科学基金项目，博士后研究人员落户黑龙江科研启动基金，哈尔滨市优秀学科带头人基金 |
| 摘 要： | 根据Genbank中提交的云芝(Coriolus versicolor (L.) Quel.)漆酶(lcc1)基因cDNA序列设计引物,利用RT-PCR获得云芝漆酶基因cDNA完整编码框,与GenBank中云芝漆酶基因同源性为96%;以pCAMBIA1381xb为初始载体,利用引物两端的酶切位点,将克隆得到的云芝漆酶基因cDNA序列分别连接到香菇gpd启动子Lg1和Lg2的下游,构建成香菇启动子引导的云芝漆酶基因真核表达载体pC-XG1-YZ-Lac和pC-XG2-YZ-Lac,利用冻融法将其转到了农杆菌EHA105中. |
| 关 键 词： | 白腐菌 漆酶 GPD启动子 表达载体构建 |
Construction of Eukaryotic Expression Vector of Laccase Gene (lcc1) from Coriolus versicolor under the Control of Lentinus edodes GPD Promoter |
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| Deng Xun,Song Ruiqing,Song Xiaoshuang,Min Kai,Sun Haizhen.Construction of Eukaryotic Expression Vector of Laccase Gene (lcc1) from Coriolus versicolor under the Control of Lentinus edodes GPD Promoter[J].Journal of Northeast Forestry University,2009,37(9). | |
| Authors: | Deng Xun Song Ruiqing Song Xiaoshuang Min Kai Sun Haizhen |
| Institution: | Deng Xun(Heilongjiang Forest Protection Institute,Harbin 150040,P.R.China),Song Ruiqing(Northeast Forestry University),Song Xiaoshuang(Heilongjiang Forest Protection Institute),Min Kai,Sun Haizhen(Northeast Forestry University) |
| Abstract: | A pair of primers was designed according to the reported cDNA sequence of laccase gene,and the total RNA was extracted from mycelium of Coriolus versicolor.An amplification product about 1 563 bp was obtained by RT-PCR reaction.Sequence analysis showed that the cloned cDNA shared a 96 percent homology in comparison with the reported data.Two express vectors,pC-XG1-YZ-Lac and pC-XG2-YZ-Lac,were constructed by restriction enzyme digestion and legation using pCAMBIA1 381 xb as initial vector,and in these two v... |
| Keywords: | White rot fungi Laccase GPD promoters Vector construction |
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