| 瓦氏黄颡鱼脂肪酸结合蛋白基因cDNA片段的克隆及分析 |
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| 引用本文： | 覃川杰,陈立侨,李二超,禹娜,杨莹,姜雪芹.瓦氏黄颡鱼脂肪酸结合蛋白基因cDNA片段的克隆及分析[J].福建农林大学学报(自然科学版),2012,41(3):259-265. |
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| 作者姓名： | 覃川杰 陈立侨 李二超 禹娜 杨莹 姜雪芹 |
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| 作者单位： | 1. 华东师范大学生命科学学院,上海200062;内江师范学院生物系,四川内江641000
2. 华东师范大学生命科学学院,上海,200062 |
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| 基金项目： | 国家"973"计划资助项目，国家科技支撑计划课题资助项目，公益性行业(农业)科研专项资助项目，内江师范学院博士基金资助项目 |
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| 摘 要： | 采用简并引物扩增得到瓦氏黄颡鱼肝型和心型脂肪酸结合蛋白cDNA片段,长度分别为335、425 bp;获得的111、114个氨基酸残基与鲤鱼(Cyprinus carpio)肝型、斜齿鳊(Rutilus rutilus)心型脂肪酸结合蛋白的氨基酸序列同源性分别达到81%和85%,表明克隆所得序列均为瓦氏黄颡鱼脂肪酸结合蛋白.定量PCR分析表明,肝脏和腹腔脂肪组织均是肝型和心型脂肪酸结合蛋白表达的主要场所.
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| 关 键 词： | 脂肪酸结合蛋白 瓦氏黄颡鱼 cDNA |
Molecular cloning and analysis of fatty acid binding protein partial cDNA in darkbarbel catfish, Pelteobagrus vachelli |
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| QIN Chuan-jie
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CHEN Li-qiao
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LI Er-chao
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YU Na
,
YANG Ying
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JIANG Xue-qin.Molecular cloning and analysis of fatty acid binding protein partial cDNA in darkbarbel catfish, Pelteobagrus vachelli[J].Journal of Fujian Agricultural and Forestry University,2012,41(3):259-265. |
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| Authors: | QIN Chuan-jie CHEN Li-qiao LI Er-chao YU Na YANG Ying JIANG Xue-qin |
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| Institution: | 1 (1.College of Life Science,East China Normal University,Shanghai 200062,China;2.Department of Life Science,Neijiang Normal University,Neijiang,Sichuan 641000,China) |
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| Abstract: | The liver and heart fatty acid binding-protein(FABP) were amplified with degenerated primers from darkbarbel catfish,Pelteobagrus vachelli.Part of the liver FABP consisted of 335 bp encoded a protein of 111amino acids,and had an overall identity of 81% in comparison with that of Cyprinus carpio.Part of the heart FABP consisted of 425 bp with 26 bp 3′-untranslated region,encoded a protein of 114 amino acids,had an overall identity of 85% in comparison with that of Rutilus rutilus.Quantitative PCR analysis showed that liver and heart FABP were mainly expressed in liver and adipose tissues. |
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| Keywords: | fatty acid binding protein Pelteobagrus vachelli cDNA |
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