| 靶标抗性点突变基因型检测技术 |
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| 引用本文： | 尚金燕,唐振华,张传溪.靶标抗性点突变基因型检测技术[J].农药学学报,2005,7(3):193-200. |
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| 作者姓名： | 尚金燕 唐振华 张传溪 |
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| 作者单位： | 1.浙江大学 应用昆虫学研究所,浙江 杭州 310029 |
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| 基金项目： | 国家重点基础研究发展计划项目（“973”项目） （2003CB114403）; 国家自然科学基金项目（320230070） . |
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| 摘 要： | 杀虫剂的不合理使用导致害虫的抗药性日趋严重。抗药性是一种涉及一个或几个昆虫基因的遗传特征。靶标抗性通常与点突变相关,对抗性相关的点突变的快速检测诊断是抗性治理的基础之一。评述了5种基因型检测技术——单链构象多态性、固相微型测序、双向等位特异PCR扩增、实时荧光定量PCR和焦磷酸测序的优缺点及其在突变检测中的应用。
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| 关 键 词： | 靶标抗性 点突变 基因型检测技术 |
| 文章编号： | 1008-7303（2005）03-0193-08 |
| 收稿时间： | 2005-04-05 |
| 修稿时间： | 2005-07-14 |
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| SHANG Jin-yan,TANG Zhen-hua and ZHANG Chuan-xi.[J].Chinese Journal of Pesticide Science,2005,7(3):193-200. |
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| Authors: | SHANG Jin-yan TANG Zhen-hua and ZHANG Chuan-xi |
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| Institution: | 1.Institute of Applied Entomology, Zhejiang University, Hangzhou 310029,China2.Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032,China |
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| Abstract: | Overapplication of insecticides made the insect pests serious resistance. Insecticide resistance is an inherited characteristic involving in one or more insect genes. Target resistance is usually (associated) with point mutation(s). Identifying the point mutation(s) is very important in resistance management. Comments on five genotyping techniques and their application in the identification of point mutation, (including) single-stranded conformational polymorphism, solid-phase minisequencing, bi-directional PCR amplification of specific alleles,real-time fluores cence quantitative polymerase chain reaction and pyrosequencing, were presented. |
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| Keywords: | target resistance point mutation genotyping techniques |
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