| 福建省福清地区马铃薯病毒病病原的分子检测 |
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| 引用本文： | 杨小龙,陈细红,蔡 伟,高芳銮,沈建国,吴祖建.福建省福清地区马铃薯病毒病病原的分子检测[J].植物保护,2019,45(3):201-205. |
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| 作者姓名： | 杨小龙 陈细红 蔡 伟 高芳銮 沈建国 吴祖建 |
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| 作者单位： | 1.福建农林大学植物保护学院, 福州 350002; 2. 福建出入境检验检疫局检验检疫技术中心, 福州 350001; 3. 福清出入境检验检疫局, 福州 350300 |
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| 基金项目： | 国家重点研发计划(2016YFF0203203); 福州市科技计划(2013-N-54) |
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| 摘 要： | 2017年调查福建福清地区马铃薯病毒病的发生情况,以明确该地区马铃薯主要病毒病原。共采集了46份疑似感染病毒的马铃薯植株,提取总RNA,利用RT-PCR技术进行分子检测,结果表明,福清地区危害马铃薯的病毒有马铃薯Y病毒Potato virus Y(PVY)、马铃薯卷叶病毒Potato leaf roll virus(PLRV)、马铃薯S病毒Potato virus S(PVS),检出率分别为56.52%、17.39%和10.87%,以PVY检出率最高,说明PVY是危害该地区马铃薯样品的主要病毒病原。通过病毒复合侵染进行分析,发现该地区存在病毒复合侵染马铃薯现象。研究结果可为福清地区马铃薯种薯的引进和病毒病害防治提供参考依据。
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| 关 键 词： | 福清地区 马铃薯病毒 RT-PCR 检测与鉴定 |
| 收稿时间： | 2018/3/1 0:00:00 |
| 修稿时间： | 2018/7/29 0:00:00 |
Molecular detection of potato virus pathogens in Fuqing area ofFujian province |
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| YANG Xiaolong,CHEN Xihong,CAI Wei,GAO Fangluan,SHEN Jianguo,WU Zujian.Molecular detection of potato virus pathogens in Fuqing area ofFujian province[J].Plant Protection,2019,45(3):201-205. |
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| Authors: | YANG Xiaolong CHEN Xihong CAI Wei GAO Fangluan SHEN Jianguo WU Zujian |
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| Institution: | 1. College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2. Inspection & Quarantine Technology Center of Fujian Entry Exit Inspection and Quarantine Bureau, Fuzhou 350001, China; 3. Fuqing Entry Exit Inspection and Quarantine Bureau, Fuzhou 350300, China |
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| Abstract: | The aim of this study is to investigate the occurrence of potato viruses in Fuqing area, Fujian province, and to diagnose the main viruses causing major diseases. In 2017, 46 infected potato plant samples were collected from Fuqing area, and the total RNA was extracted from the infected potato leaves. From these samples, PVY, PLRV and PVS were detected via the conventional RT PCR techniques, and the detection rates were 56.52%, 17.39% and 10.87%, respectively, while other viruses were not detected. The analysis of complex infection showed that complex infection existed in potato plants in this area. This study can provide reference for the introduction of seed potato and the management strategy of virus diseases control in Fuqing area. |
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| Keywords: | Fuqing area potato virus RT PCR detection and identification |
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