| 衰老对斑马鱼呼吸代谢和游泳行为的影响 |
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| 引用本文： | 高泉,吕为群.衰老对斑马鱼呼吸代谢和游泳行为的影响[J].上海海洋大学学报,2019,28(2):200-210. |
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| 作者姓名： | 高泉 吕为群 |
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| 作者单位： | 上海海洋大学 水产科学国家级实验教学示范中心, 上海 201306;上海海洋大学 水产种质资源发掘与利用教育部重点实验室, 上海 201306,上海海洋大学 水产科学国家级实验教学示范中心, 上海 201306;上海海洋大学 水产种质资源发掘与利用教育部重点实验室, 上海 201306;上海海洋大学 科技部海洋生物科学国际联合研究中心, 上海 201306 |
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| 基金项目： | 国家自然科学基金（31572599，31072228，41376134） |
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| 摘 要： | 以模式生物斑马鱼作为实验对象,首先从躯体形态和繁殖能力上对6月龄与30月龄斑马鱼的特征进行了区分,而后分别比较了6月龄和30月龄斑马鱼在昼夜节律、应激行为和呼吸代谢方面的差异,研究衰老对斑马鱼代谢和行为的影响。结果显示:30月龄斑马鱼脊柱弯曲,并且在产卵量、受精率、孵化率和成活率等方面均低于6月龄斑马鱼,显示出明显的衰老特征。6月龄斑马鱼昼夜间的游泳行为具有明显的节律性,与30月龄斑马鱼相比,其白天活动量大,夜晚静息,昼夜间游泳行为具有显著差异。当遭受驱逐刺激后,6月龄斑马鱼的快速游泳距离、快速游泳时间和平均游泳速度均高于30月龄斑马鱼(P 0. 05);当遭受光照刺激后,6月龄斑马鱼的快速游泳距离和平均游泳速度高于30月龄斑马鱼(P 0. 05)。应激行为实验结果表明:衰老斑马鱼的应激游泳能力显著低于年轻斑马鱼。30月龄斑马鱼的标准代谢率(SMR)、最大代谢率(MMR)和代谢范围(MS)低于6月龄斑马鱼(P 0. 05),与应激游泳行为的差异一致。繁殖、代谢和应激游泳行为是鱼类重要的生理活动,研究衰老与年轻斑马鱼的生理行为,有助于进一步理解衰老对机体生理功能的影响,并为深入探究衰老对斑马鱼代谢和行为影响的内在机制奠定基础。
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| 关 键 词： | 斑马鱼 衰老 昼夜节律 应激行为 呼吸代谢 |
| 收稿时间： | 2018/5/8 0:00:00 |
| 修稿时间： | 2018/5/18 0:00:00 |
Effects of aging on the respiratory metabolism and swimming behavior of zebrafish |
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| GAO Quan and L&#; Weiqun.Effects of aging on the respiratory metabolism and swimming behavior of zebrafish[J].Journal of Shanghai Ocean University,2019,28(2):200-210. |
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| Authors: | GAO Quan and L&#; Weiqun |
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| Institution: | National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China and National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China;International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China |
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| Abstract: | Aging is a series of complex life processes that occurs in living organisms and usually leads to a series of degenerative manifestations in reproduction, metabolism, stress, and exercise behavior. In this experiment, the model organism zebrafish was used as an experimental animal. The characteristics of 6-month and 30-month zebrafish were distinguished based on the body morphology and reproductive ability, and the circadian rhythm, stress behavior and respiratory metabolism of 6-month and 30-month zebrafish were compared, in order to investigate the effects of aging on metabolism and behavior of zebrafish. The results showed that the spine of 30-month zebrafish was curved and the reproductive performance of old fish was lower than the 6-month zebrafish in terms of fecundity, fertilization rate, hatching rate and survival rate, showing obvious aging characteristics. The swimming behavior of the 6-month zebrafish dispalys an obvious circadian rhythm. Compared with the 30-month zebrafish, 6-month zebrafish is active in the daytime, and always rest at night. The swimming behavior in the daytime and nighttime is significantly different. When 6-month zebrafish suffered from chase-stress, their large movement distance, large movement time and average swimming speed were higher than those of 30-month zebrafish (P<0.05). When exposed to light-stress, the large movement distance and average swimming speed of 6-month zebrafish were higher than those of 30-months zebrafish (P<0.05). The results of stress behavior showed that the stress response ability in aging zebrafish is significantly weaker than that of young zebrafish. The standard metabolic rate (SMR), maximum metabolic rate (MMR), and metabolic space (MS) of 30-month zebrafish were lower than those of 6-month zebrafish (P<0.05), which was consistent with the difference in stressed swimming behavior. Reproduction, metabolism and stress behavior are important physiological activities of fish, and investigating these physiological responses and behaviors in aging and young zebrafish can help to understand the effects of aging on the physiological functions of organism, and furthermore, the molecular mechanism of metabolism and behavior associated with aging. |
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| Keywords: | zebrafish aging circadian rhythm stress behavior respiratory metabolism |
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