| 花药培养与麦谷蛋白亚基分子标记结合选育小麦新品种的研究 |
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| 引用本文： | 王 炜,陈 琛,叶春雷,杨随庄,欧巧明,罗俊杰.花药培养与麦谷蛋白亚基分子标记结合选育小麦新品种的研究[J].麦类作物学报,2019(3):277-282. |
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| 作者姓名： | 王 炜 陈 琛 叶春雷 杨随庄 欧巧明 罗俊杰 |
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| 作者单位： | （1.甘肃省农业科学院生物技术研究所，甘肃兰州 730070；2.西南科技大学生命科学与工程学院,四川绵阳 621010） |
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| 基金项目： | 甘肃省农业生物技术研究与应用开发项目（GNSW-2014-15）；甘肃省农科院科技创新工程学科团队项目（2014GAAS06，2015GAAS02）；甘肃省现代农业产业技术体系项目（GARS-01-03） |
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| 摘 要： | 为快速获得携带麦谷蛋白优质亚基基因的小麦新品种,提高小麦的品质育种技术水平,利用引进的矮败材料与和尚头、甘春20号、临麦34号等10个不同品种(系)杂交,并对杂交后代进行了花药培养,获得了115份花培株系;利用PCR对花培后代株系及杂交亲本进行了优质贮藏蛋白亚基分子标记检测,3个HMW-GS为 Bx7、 Bx14、 Dx5,3个LMW-GS为 Glu-A3ac、 Glu-A3d、 Glu-B3b。结果表明,在115份花培材料中, Bx7的出现频率最高,为94.78%,其余依次为 Glu-A3ac、 Dx5、 Bx14、 Glu-A3d和 Glu-B3b;获得了44份聚合4个亚基以上的材料;结合农艺性状鉴定,筛选出了3份综合性状优异的小麦新品系AB158、AB167和AB332。本研究将花培育种技术、分子标记辅助选择技术及矮败小麦育种技术进行了有机结合,其结果可为提升小麦品质育种技术水平提供参考。
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| 关 键 词： | 小麦 花药培养 分子标记 麦谷蛋白亚基 |
Studies on Wheat Breeding by Combination of Anther Culture and Glutenin-Subunit Molecular Marker Techniques |
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| WANG Wei,CHEN Chen,YE Chunlei,YANG Suizhuang,OU Qiaoming,LUO Junjie.Studies on Wheat Breeding by Combination of Anther Culture and Glutenin-Subunit Molecular Marker Techniques[J].Journal of Triticeae Crops,2019(3):277-282. |
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| Authors: | WANG Wei CHEN Chen YE Chunlei YANG Suizhuang OU Qiaoming LUO Junjie |
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| Abstract: | To breed wheat new variety with elite glutenin subunits and improve the technical level of breeding for wheat quality improvement, the hybrid progenies of an introduced dwarf-sterile wheat crossed with 10 different varieties, such as Heshangtou, Ganchun 20, Linmai 34, etc. were anther-cultured and 115 anther-cultured plant lines were obtained, which were identified by using three HMW-GS(Bx7, Bx14, and Dx5) and three LMW-GS(Glu-A3ac, Glu-A3d, and Glu-B3b) molecular markers to determine the presence of these subunits in the anther-cultured progenies and the hybrid parents. Results showed that the frequency of Bx7 is the highest among these 115 materials, accounting for 94.78%, followed by the remaining alleles:Glu-A3ac, Dx5, Bx14, Glu-A3d and Glu-B3b; 44 materials contain at least four subunits; three new wheat lines with elite comprehensive traits were selected by combining with determination of agronomic traits in the field. The anther-cultured breeding technology, molecular marker technology, and dwarf-sterile wheat breeding technology were combined in this research, and the results can provide reference for improving the technical level of wheat quality breeding. |
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| Keywords: | Wheat Anther culture Molecular marker Glutenin subunit |
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