Primary studies on tissue culture from mature embryos in diploid and tetraploid wheat |
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| Authors: | Ruiming Bi Honggang Wang |
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| Institution: | (1) Department of Life Science of Heze University, Heze, 274015, China;(2) Subcenter of National Wheat Improvement Center, the National Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an, 271018, China |
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| Abstract: | Using mature embryos (MEs) as the explants, the callus induction, embryogenic callus differentiation, plantlet regeneration
and culture efficiency in diploid and tetraploid wheat of four genotypes were studied. The tested four genotypes wheat included
cultivable emmer wheat (Triticum dicoccum Schuble), durum wheat (Triticum durum Desf.) and the common wheat progenitors Triticum dicoccoides and Triticum aegilopides. Results indicated that there were significant differences in the efficiency of callus induction, callus differentiation
and plant regeneration among the tested genotypes. The efficiency of differentiation and regeneration shows strong genotype
dependence. The rates of callus induction, embryogenic callus differentiation, plantlet regeneration and culture efficiency
respectively were 95.00%, 90.00%, 32.40%, and 27.70% in cultivable emmer wheat, which were significantly higher than other
tested genotypes. Therefore, this study has provided a basis for genetic transformation, gene cloning and molecular plant
breeding in wheat and other related species.
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Translated from Molecular Plant Breeding, 2007, 5(4): 480–484 译自: 分子植物育种] |
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| Keywords: | diploid wheat tetraploid wheat mature embryo tissue culture |
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