| 蛹虫草液体深层发酵的研究 |
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| 引用本文： | 秦秀丽,杨国会,李风林.蛹虫草液体深层发酵的研究[J].北方园艺,2010(23):167-170. |
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| 作者姓名： | 秦秀丽 杨国会 李风林 |
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| 作者单位： | 吉林农业科技学院,吉林,吉林,132101;吉林农业科技学院,吉林,吉林,132101;吉林农业科技学院,吉林,吉林,132101 |
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| 基金项目： | 吉林省教育厅"十一五"科学技术研究资助项目 |
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| 摘 要： | 以蛹虫草菌丝体生物量为指标,运用单因素对比试验和正交实验,对蛹虫草液体培养基的碳、氮营养源以及最佳配方和培养条件进行了优化研究。结果表明:筛选出蛹虫草液体培养基最佳碳源为蔗糖,其次为麦芽糖;最佳氮源为酵母膏,其次为蛋白胨。筛选出蛹虫草液体培养基适宜配方为:蔗糖3%、酵母膏3%、KH2PO40.1%、MgSO40.15%;液体培养适宜条件的温度为24℃,pH 5.5,接种量为15%,摇瓶转速为140 r/min,发酵罐通气量为1∶1.2~1∶1.6V/(V.min)。
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| 关 键 词： | 蛹虫草 液体发酵 培养基 菌丝体 生物量 |
Submerged Fermentation of Cordyceps militaris |
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| QIN Xiu-li,YANG Guo-hui,LI Feng-lin.Submerged Fermentation of Cordyceps militaris[J].Northern Horticulture,2010(23):167-170. |
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| Authors: | QIN Xiu-li YANG Guo-hui LI Feng-lin |
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| Institution: | QIN Xiu-li,YANG Guo-hui,LI Feng-lin(Jilin Agricultural Science and Technology College,Jilin,Jilin 132101) |
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| Abstract: | In this experiment,the best formula of carbon,nitrogen nutrition source and culture conditions on liquid medium of Cordyceps militaris were optimized using cordyceps militaris mycelia biomass as target by single factor contrast experiment and orthogonal test.The optimal liquid medium of Cordyceps militaris were selected,sucrose was the best carbon source,followed by maltose,Yeast extract was the best nitrogen source,followed by peptone.The suitable formula for liquid medium of Cordyceps militaris were confi... |
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| Keywords: | Cordyeeps militaris fermentation medium mycelia biomass |
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