| 油菜种子中烯丙基硫苷的HLPC检测体系建立及其含量测定 |
| |
| 引用本文： | 吴媛媛,李胜,刘媛,张青松,马绍英.油菜种子中烯丙基硫苷的HLPC检测体系建立及其含量测定[J].甘肃农业大学学报,2009,44(5). |
| |
| 作者姓名： | 吴媛媛 李胜 刘媛 张青松 马绍英 |
| |
| 作者单位： | 1. 甘肃农业大学生命科学技术学院,甘肃,兰州,730070
2. 兰州汇通生物科技有限公司,甘肃,兰州,730000 |
| |
| 基金项目： | 科技部中小企业创业基金，甘肃省科技攻关项目，甘肃省高校研究生导师项目，兰州市高层次人才资助项目 |
| |
| 摘 要： | 实验以油菜种子为材料,建立了其中所含烯丙基硫苷的HPLC检测体系,并对不同油菜种子中烯丙基硫苷的含量进行了测定.结果表明,烯丙基硫苷HPLC分离检测的最佳条件为:流动相:甲醇∶水(0.005mol.L-1KCl)=9∶91;流速:0.5mL.min-1;柱温:30℃;检测波长:230nm.推广品种‘中陇油5’号烯丙基硫苷含量最高,达到8.677μmol.g-1;‘绵杂04-20’种子烯丙基硫苷含量只有0.032μmol.g-1.杂交品系油菜种子中的烯丙基硫苷含量普遍高于推广品种,最高达11.705μmol.g-1.
|
| 关 键 词： | 油菜种子 烯丙基硫苷 HPLC |
Determination of sinigrin in different kinds of rapeseed by HPLC |
| |
| WU Yuan-yuan,LI Sheng,LIU Yuan,ZHANG Qing-song,MA Shao-ying.Determination of sinigrin in different kinds of rapeseed by HPLC[J].Journal of Gansu Agricultural University,2009,44(5). |
| |
| Authors: | WU Yuan-yuan LI Sheng LIU Yuan ZHANG Qing-song MA Shao-ying |
| |
| Institution: | WU Yuan-yuan,LI Sheng,LIU Yuan,ZHANG Qing-song,MA Shao-ying(College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China,Lanzhou Huitong Biological Science & Technology Ltd.,Lanzhou 730000,China) |
| |
| Abstract: | An method based on the HPLC for the determination of sinigrin in rapeseed was established. HPLC analysis was performed on an YWG-C18 column (250 mm×4. 6 mm 5 μm). The mobile phase con-sisted of H2O(0. 005 mol · L~(-1) KC1) : methanol = 91: 9. The flow rate: 0. 5 mL · min~(-1), column tempera-ture: 30 ℃,HPLC chromatogram ;230 nm. Contents of sinigrin in 20 kinds of rapeseed were determined. Concentration of sinigrin in the rapeseed cultivar 'Longyou No. 5' was as high as 8. 677 μmol ·g~(-1), while the cultivar 'Mianza 04-20' was only 0. 032μrnol · g~(-1). Results of cross-breeds were generally higher than commercial varieties. |
| |
| Keywords: | HPLC |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
