| 李与杏种间杂种的RAPD和SSR分析 |
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| 引用本文： | 杨红花,秦宏伟,米玛潘多,肖军.李与杏种间杂种的RAPD和SSR分析[J].安徽农业科学,2013(2):497-498,605. |
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| 作者姓名： | 杨红花 秦宏伟 米玛潘多 肖军 |
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| 作者单位： | 泰山学院生物与酿酒工程学院,山东泰安,271021;泰山学院机械与工程学院,山东泰安,271021;西藏自治区高原生物研究所,西藏拉萨,850001 |
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| 基金项目： | 山东省自然科学基金(Q2008D01);山东省高校科研发展计划项目(J09LC54) |
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| 摘 要： | 目的]探讨杂种苗与亲代之间的遗传变异及其真伪性,获取快速鉴定杂种的方法。方法]利用RAPD和SSR分子标记技术对凯特杏和莫尔特尼李的种间杂交种进行分析。结果]叶片形态、RAPD和SSR技术对杂种的鉴定分析,表明子代与亲代电泳图谱之间存在差异,所获杂种为真杂种。结论]将RAPD及SSR技术结合可有效鉴定李杏种间杂交种,是一种快速鉴定杂种的方法,可缩短育种周期,提高育种效率。
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| 关 键 词： | 李 杏 种间杂交 RAPD SSR |
Identification of Hybrids between Prunus salicina and Armeniaca vulgaris by RAPD and SSR |
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| Institution: | YANG Hong-hua et al(Institute of Biological & Wine Engineering,Tai’an,Shandong 271021) |
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| Abstract: | Objective] This study aimed to investigate the genetic variation between parents and hybrids,and estimate they were true or not,to find a rapid identification method.Method] The progenies between Armeniaca vulgaris Katy and Prunus salicina Moriteni were analyzed by means of RAPD and SSR molecular markers.Result] Electrophoresis differences between progenies and parents were observed,indicating that hybrid seedlings obtained were real.Conclusion] The combination of the above method is effective and fast to identify the hybrids,and can shorten the breeding period. |
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| Keywords: | Prunus salicina Armeniaca vulgaris Interspecific cross RAPD SSR |
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