| 天仙果根总黄酮提取工艺研究 |
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| 引用本文： | 王喜周,应跃跃,张昊,姚晓伟,陈学智.天仙果根总黄酮提取工艺研究[J].安徽农业科学,2013(2):601-603. |
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| 作者姓名： | 王喜周 应跃跃 张昊 姚晓伟 陈学智 |
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| 作者单位： | 丽水学院医学院,浙江丽水,323000;丽水市质量技术监督检测院,浙江丽水,323000;景宁县人民医院,浙江丽水31000 |
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| 基金项目： | 浙江省科技厅公益项目(2011C23128);2010年度丽水学院重点科研项目(kz201018);2011年度丽水市科技局公益项目(20111JYZB20) |
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| 摘 要： | 目的]优选天仙果根中总黄酮的超声辅助提取工艺。方法]以总黄酮的含量为指标,采用单因素试验考察乙醇浓度、料液比、提取时间和提取次数对天仙果根部总黄酮得率的影响,并采用正交试验优选天仙果根总黄酮的超声辅助提取工艺。结果]4个因素对天仙果根部总黄酮提取得率的影响大小顺序为:料液比>乙醇浓度>提取时间>提取次数;最佳提取工艺为:提取溶剂乙醇的浓度为50%,料液比1∶20(W/V,g/ml,下同),提取2次,每次60 min;在此条件下,天仙果根部总黄酮得率为27.83 mg/g。结论]提取工艺合理,测定方法简单、快速、准确,为天仙果深度开发提供了科学依据。
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| 关 键 词： | 天仙果(Ficus erecta Thunb.var.beecheyana(Hook.et Arn.)King) 总黄酮 提取工艺 紫外分光光度法 |
Extraction and Determination of Total Flavonoids from Ficus beecheyana Radix |
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| Institution: | WANG Xi-zhou et al(Medical College of Lishui University,Lishui,Zhejiang 323000) |
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| Abstract: | Objective] To optimum ultrasonic-assisted extraction technique of total flavonoids in radix of Ficus beecheyana.Method] Effects of ethanol concentration,solid-liquid ratio,extraction time and times on yield of total flavonoids were investigated by using orthogonal test,and the contents were determined by UV spectrophotometry.Result] The order of influencing factors is： solid-liquid ratio ethanol concentration extraction time extraction times;the optimal extraction technique is： ethanol concentration 50%,solid-liquid ratio 1∶20（W/V,g/ml,the same as follows）,extraction 2 times and extraction time 60 min.Under these optimum conditions,the extraction efficiency of total flavonoids reached as high as 27.83 mg/g.Conclusion] The extraction technique is proper,the determination method is simple,rapid and accurate,which will provide a scientific basis for deeply utilization of Ficus beecheyana. |
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| Keywords: | Ficus beecheyana Total flavonoids Extraction technique UV spectrophotometry |
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