| 嘌呤核苷类核成熟抑制剂对延边奶山羊卵母细胞体外成熟的影响 |
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| 引用本文： | 柳海星,尹多,李钟淑,高见红,方南洙.嘌呤核苷类核成熟抑制剂对延边奶山羊卵母细胞体外成熟的影响[J].安徽农业科学,2013,41(3):1114-1116. |
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| 作者姓名： | 柳海星 尹多 李钟淑 高见红 方南洙 |
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| 作者单位： | 延边大学农学院,吉林延吉,133002;延边朝鲜族自治州畜牧总站,吉林延吉,133002 |
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| 基金项目： | 吉林省科技厅重点项目(20100228) |
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| 摘 要： | 目的]提高卵母细胞体外成熟率及其后续发育能力。方法]以延边奶山羊为试验对象,活体采卵所获得的卵母细胞为材料,利用核成熟抑制剂HX和IBMX分别进行体外成熟培养,研究嘌呤核苷类核成熟抑制剂对延边奶山羊卵母细胞体外成熟的影响。结果]所有浓度HX和IBMX均可有效维持卵母细胞停留在GV期且呈剂量依赖性逐渐升高。最适宜延边奶山羊卵母细胞成熟的HX和IB-MX添加浓度分别为2和0.2 mmol/L。结论]该研究可为延边奶山羊的转基因和体细胞克隆试验等提供稳定的成熟卵母细胞来源。
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| 关 键 词： | 核成熟抑制剂 延边奶山羊 卵母细胞 体外成熟 |
Effect of Purine Nucleoside Nuclear Maturation Inhibitors on Oocyte Maturation in vitro of Yanbian Dairy Goat |
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| Institution: | LIU Hai-xing et al(Agricultural College of Yanbian University,Yanji,Jilin 133002) |
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| Abstract: | Objective ] The research aimed to improve the maturation rate of oocyte in vitro and its subsequent development. Method ] With Yanbian dairy goat as test object, the collected oocytes by OPU was used as materials. Nuclear maturation inhibitors HX and IBMX were used in maturation and culture in vitro. The effects of purine nucleoside nuclear maturation inhibitors on the maturation of oocyte in vitro were stud- ied. Result] All concentrations of HX and IBMX could effectively maintain oocytes in GV stage and showed a gradual increase with the dose. The optimum concentrations of HX and IBMX for the oocyte maturation of Yanbian dairy goat were 2 and 0.2 mmol/L respectively. Conclu- sion ] The research could orovide stable sources of mature oocvtes for the gene transfer and somatic cell cloning tests. |
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| Keywords: | Nuclear matruation inhibitor Yanbian dairy gota Oocyte Maturation in vitro |
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