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18种中草药对大肠杆菌抑菌效果的研究
引用本文:赵鑫荟,艾启俊.18种中草药对大肠杆菌抑菌效果的研究[J].安徽农业科学,2013(2):599-600.
作者姓名:赵鑫荟  艾启俊
作者单位:北京农学院食品科学学院,北京,102206;北京农学院食品科学学院,北京,102206
摘    要:目的]研究鹿蹄草(WINTERGREEN)、功劳叶(CHINESE HOLLY LEAF)、金樱子(CHEROKEE ROSE FRUIT)和大黄(RHEUMOFFICINALE)等18种中草药提取物的抑菌作用。方法]采用水煎法提取中草药的有效成分,以大肠杆菌作为供试菌,利用滤纸片法测定中草药提取物的抑菌作用,并用2倍稀释法测定抑菌效果最好的4种中草药的最小抑菌浓度。结果]18种中草药中有15种对大肠杆菌有抑制作用;其中鹿蹄草的最小抑菌浓度为31.25 mg/ml,功劳叶、金樱子和大黄的最小抑菌浓度均为62.50 mg/ml。结论]鹿蹄草对大肠杆菌具有较好的抑制效果。

关 键 词:大肠杆菌  中草药  抑菌作用

Studies on Inhibitory Effect of 18 Kinds of Chinese Herbal Medicine on Escherichia coli
Institution:ZHAO Xin-hui et al(Department of Food Science,Beijing University of Agriculture,Beijing 102206)
Abstract:Objective] The purpose was to investigate the inhibitory effects of 18 kinds of Chinese herbal medicine including WINTERGREEN,CHINESE HOLLY LEAF,CHEROKEE ROSE FRUIT,RHEUM OFFICINALE.Method] Water decoction method was adopted to extract the active ingredients from Chinese herbal medicine,whose bacteriostatic effect was then determined by filter paper tablet method with Escherichia coli as the indicator bacteria.Double dilution method was used to determine the minimum inhibition concentrations of four kinds of Chinese herbal medicine,whose bacteriostatic effect was best.Result] Among the 18 kinds of Chinese herbal medicine,the extracts from 15 kinds of medicine had Inhibitory effect on Escherichia coli.The minimum inhibition concentration of Wintergreen was 31.25 mg/ml and Chinese holly leaf,Cherokee rose fruit,rheum officinale was all 62.50 mg/ml.Conclusion] The Wintergreen had a good inhibition effect to Escherichia coli.
Keywords:Escherichia coli  Chinese herbal medicine  Bacteriostatic effect
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