| 猪肌生成抑制素成熟蛋白编码序列真核表达载体的构建 |
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| 引用本文： | 李树伟,欧阳红生,张英霞,吕文发,李慎涛,张永亮,张玉静.猪肌生成抑制素成熟蛋白编码序列真核表达载体的构建[J].中国兽医学报,2003,23(6):610-612. |
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| 作者姓名： | 李树伟 欧阳红生 张英霞 吕文发 李慎涛 张永亮 张玉静 |
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| 作者单位： | 解放军军需大学,军事兽医系,吉林,长春,130062 |
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| 基金项目： | 国家自然科学基金资助项目 (3 0 0 70 5 63 )，军队医药卫生青年基金资助项目(98Q0 79) |
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| 摘 要： | 从猪肌生成抑制素(MSTN)cDNA序列中设计引物。以pMD18-T-MSTN质粒为模板，PCR扩增猪MSTN成熟蛋白编码序列，该片段全长1095bp。将所得片段与pMD18-T载体连接，构建重组质粒pMT—mpMSTN，将其转化到大肠杆菌DH5a中，成功地筛选到阳性克隆。双酶切重组质粒pMT—mpMSTN和真核表达载体pcDNA3．1，连接目的片段与载体pcDNA3．1，转化大肠杆菌DH5a，经酶切、PCR及测序分析，表明成功地构建了真核表达质粒pcDNA3．1-mpMSTN。
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| 关 键 词： | 猪肌生成抑制素 成熟蛋白 编码序列 真核表达 骨骼肌生长 负调控因子 |
| 文章编号： | 1005-4545(2003)06-0610-03 |
| 修稿时间： | 2002年6月10日 |
Construction of the Recombinant Eukaryotic Expression Vector of Coding Sequence of Porcine Myostatin |
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| LI Shu wei,OUYANG Hong sheng ,ZHANG Ying xia,LU Wen fa,LI Sheng tao,ZHANG Yong liang,ZHANG Yu jing.Construction of the Recombinant Eukaryotic Expression Vector of Coding Sequence of Porcine Myostatin[J].Chinese Journal of Veterinary Science,2003,23(6):610-612. |
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| Authors: | LI Shu wei OUYANG Hong sheng ZHANG Ying xia LU Wen fa LI Sheng tao ZHANG Yong liang ZHANG Yu jing |
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| Institution: | LI Shu wei,OUYANG Hong sheng *,ZHANG Ying xia,LU Wen fa,LI Sheng tao,ZHANG Yong liang,ZHANG Yu jing |
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| Abstract: | In this research,the coding sequence of MSTN mature protein was amplified by PCR from myostatin plasmid pMD18 T MSTN of a new porcine breed JunMu 1.The fragment with the expected size was cloned into vector pMD18 T and subsequently subjected to PCR,restriction endonucleases analysis and sequencing.Then,the fragment was cloned into vector pcDNA3 1 for eukaryotic expression.The coding sequcene of MSTN mature protein was coincidence with our design after PCR,restriction endonucleases analysis and sequencing.The expression of the cloned coding sequence of MSTN mature protein in eukaryotic system is under way. |
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| Keywords: | pig myostatin eukaryotic expression |
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