A high-density Diversity Arrays Technology (DArT) microarray for genome-wide genotyping in <Emphasis Type="Italic">Eucalyptus</Emphasis> |
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| Authors: | Carolina P Sansaloni César D Petroli Jason Carling Corey J Hudson Dorothy A Steane Alexander A Myburg Dario Grattapaglia René E Vaillancourt Andrzej Kilian |
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| Institution: | 1.Plant Genetics Laboratory,EMBRAPA Genetic Resources and Biotechnology - EPqB,Brasilia,Brazil;2.Dep. Cell Biology,Universidade de Brasilia,Brasília,Brazil;3.Diversity Arrays Technology Pty Ltd,Australia;4.School of Plant Science and Cooperative Research Centre for Forestry,University of Tasmania,Hobart,Australia;5.Department of Genetics,Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,Pretoria,South Africa;6.Genomic Sciences Program,Universidade Católica de Brasília - SGAN,Brasília,Brazil |
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| Abstract: | Background A number of molecular marker technologies have allowed important advances in the understanding of the genetics and evolution
of Eucalyptus, a genus that includes over 700 species, some of which are used worldwide in plantation forestry. Nevertheless, the average
marker density achieved with current technologies remains at the level of a few hundred markers per population. Furthermore,
the transferability of markers produced with most existing technology across species and pedigrees is usually very limited.
High throughput, combined with wide genome coverage and high transferability are necessary to increase the resolution, speed
and utility of molecular marker technology in eucalypts. We report the development of a high-density DArT genome profiling
resource and demonstrate its potential for genome-wide diversity analysis and linkage mapping in several species of Eucalyptus. |
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