| 牛胎儿成纤维细胞分离与体外培养 |
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| 引用本文： | 徐小明,窦忠英,华进联,葛秀国.牛胎儿成纤维细胞分离与体外培养[J].中国畜牧杂志,2004,40(10):18-21. |
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| 作者姓名： | 徐小明 窦忠英 华进联 葛秀国 |
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| 作者单位： | 西北农林科技大学陕西省干细胞工程技术研究中心,陕西杨凌,712100 |
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| 摘 要： | 本研究以 4 5d牛胎儿为材料 ,使用 0 .2 5 %胰酶 + 0 .0 4 %EDTA消化液 ,分离得到牛胎儿成纤维细胞 ,体外培养传代至第 2 8代。本实验描绘出牛胎儿成纤维细胞生长曲线 ,并发现α MEM是一种适合牛胎儿成纤维细胞生长的培养基。分离得到的牛胎儿成纤维细胞传至第 1 8代时核型仍然正常 ,冷冻、解冻后细胞可继续传代
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| 关 键 词： | 畜牧、兽医科学基础学科 胎儿成纤维细胞 分离 体外培养 牛 |
| 文章编号： | 0258-7033(2004)10-0018-03 |
| 修稿时间： | 2003年7月26日 |
Isolation and Culture of Bovine Fetal Fibroblasts |
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| XU Xiao-ming,DOU Zhong-ying,HUA Jin-lian,GE Xiu-guo.Isolation and Culture of Bovine Fetal Fibroblasts[J].Chinese Journal of Animal Science,2004,40(10):18-21. |
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| Authors: | XU Xiao-ming DOU Zhong-ying HUA Jin-lian GE Xiu-guo |
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| Institution: | XU Xiao-ming,DOU Zhong-ying~,HUA Jin-lian,GE Xiu-guo |
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| Abstract: | The major objective of this study was to isolate and culture bovine fetal fibroblasts from a 45-day-old fetus us- ing 0.25% trypsin + 0.04% EDTA dispersed liquid. These fibroblasts were cultured in vitro and passaged 28 passages. We described the growth curve of bovine fetal fibroblasts and found that α-MEM was a good cell culture medium for bovine fetal fibroblasts. There weren't changes in the chromosome number of 18 passages bovine fetal fibroblasts. Bovine fetal fibroblasts could subculture when they were cyto-preserved and thawed. |
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| Keywords: | Basal disciplines of animal and veterinary Fetal fibroblasts Isolation Culture Bovine |
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