| 野牛草种子蛋白质组双向电泳样品制备方法的分析比较 |
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| 引用本文： | 孙杰,刘永志,康俊梅,熊军波,沈静,吕会刚,孙彦.野牛草种子蛋白质组双向电泳样品制备方法的分析比较[J].安徽农业科学,2009,37(13). |
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| 作者姓名： | 孙杰 刘永志 康俊梅 熊军波 沈静 吕会刚 孙彦 |
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| 作者单位： | 内蒙古农业大学,内蒙古呼和浩特,010018;内蒙古农牧业科学院,内蒙古呼和浩特,010030;中国农业科学院北京畜牧兽医研究所,北京,100193;内蒙古农牧业科学院,内蒙古呼和浩特,010030;中国农业科学院北京畜牧兽医研究所,北京,100193;甘肃农业大学,甘肃兰州,730070;中国农业大学,北京,100191 |
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| 基金项目： | 中国农业科学院北京畜牧兽医研究所所长基金项目 |
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| 摘 要： | 目的]比较野牛草种子蛋白质的提取方法。方法]以"中坪一号"野牛草种子为试材,分别采用TCA/A(三氯乙酸/丙酮)沉淀法和Trizol法提取种子蛋白,并利用条件一致的蛋白质双向电泳体系进行分析比较。结果]TCA/A法提取的蛋白质双向电泳图谱背景噪音大,清晰度差,横向条纹较多,图谱上呈现多个形状不规则的斑点,杂色与正常蛋白质点有区别,对其他蛋白质点构成高度覆盖和遮避,影响图谱信息的准确表达;Trizol法所提取的蛋白质样品,其双向电泳图谱背景噪音小,横向条纹少,非蛋白类杂质少,无斑点间的遮避现象,蛋白质点数量增多,为野牛草种子蛋白质组定量、定性分析提供了丰富的信息。结论]与TCA/A沉淀法相比,改进的Trizol法是野牛草种子蛋白质提取的适用方法。
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| 关 键 词： | 野牛草 种子蛋白质组 蛋白质双向电泳 图谱 |
Comparative Analysis on the Preparation Methods of Bidirectional Electrophoretic Samples of Proteome from Buffalo Grass Seeds |
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| SUN Jie et al.Comparative Analysis on the Preparation Methods of Bidirectional Electrophoretic Samples of Proteome from Buffalo Grass Seeds[J].Journal of Anhui Agricultural Sciences,2009,37(13). |
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| Authors: | SUN Jie |
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| Institution: | SUN Jie et al (Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010018) |
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| Abstract: | Objective] The aim of the study was to compare the methods of extracting protein from buffalo grass seeds.Method] With seeds of buffalo grass Zhongping 1 as tested materials,the proteins were extracted from seeds by TCA/A precipitation and Trizol method and analyzed and compared by bidirectional electrophoresis system with uniform conditions of protein.Result] The background noise of bidirectional electrophoresis map of protein extracted by TCA/A method was greater,its clarity was poor,its horizontal str... |
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| Keywords: | Buffalo grass Proteome from seeds Bidirectional electrophoresis of protein Map |
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