Fertility of teleost semen as affected by dilution and storage in a seminal plasma-mimicking medium |
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| Authors: | A W Erdahl and E F Graham |
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| Institution: | University of Minnesota, 495 AnSc/VetMed Building, 1988 Fitch Avenue, St. Paul, MN 55108, U.S.A. |
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| Abstract: | Fertility trials were run with fish semen diluted in Fish Extender #6 and stored for up to 24 h at 10°C. After 30 min of storage, rainbow trout (Salmo gairdneri) and northern pike (Esox lucius) semen diluted 1:255 maintained control values of fertility. Brown trout (Salmo trutta) and pink salmon (Oncorhynchus gorbuscha) semen diluted 1:511 also maintained control values of fertility after 30 min of storage. As storage times increased, the dilution ratios that maintained control values of fertility decreased. After 24 h of storage, rainbow trout semen diluted 1:31 and brown trout semen diluted 1:63 maintained control values of fertility. A field trial with rainbow trout semen showed that semen diluted 1:8 maintained control values of fertility after 30 min of storage. Non-motile sperm cells did not significantly (P>0.05) interfere with the fertilizing ability of motile sperm cells. Calculations from the dilution trials on the number of inseminated sperm cells showed that approximately 4000–169 000 motile sperm cells/egg were required to maintain control values of fertility. |
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