| 魟鱼软骨多糖的聚丙烯酰胺凝胶电泳鉴别 |
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| 引用本文： | 王洪建,郭斌.魟鱼软骨多糖的聚丙烯酰胺凝胶电泳鉴别[J].安徽农业科学,2011(8):4734-4735. |
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| 作者姓名： | 王洪建 郭斌 |
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| 作者单位： | 辽宁医学院;辽宁医学院附属一院 |
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| 基金项目： | 辽宁省教育厅基金项目(202173371) |
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| 摘 要： | 目的]用聚丙烯酰胺凝胶电泳对魟鱼软骨多糖（RCG）进行定性鉴别。方法]利用纯化RCG,进行聚丙烯酰胺凝胶电泳。结果]RCG经纯化,基本实现了多糖的分离;根据聚丙烯酰胺凝胶电泳迁移率,可明显鉴别出魟鱼RCG。结论]该法重现性较好,简单易行,可用于魟鱼RCG的定性鉴别。
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| 关 键 词： | 魟鱼软骨多糖 纯化 聚丙烯酰胺凝胶电泳 鉴别 |
Identification of Ray Cartilage Glycosaminoglycans by Polyacrylamide Gel Electrophoresis |
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| Institution: | WANG Hong-jian et al(Liaoning Medical University,Jinzhou,Liaoning 121001) |
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| Abstract: | Objective] This paper aims to study the feasibility of qualitative determination of Ray Cartilage Glycosaminoglycans(RCG) by polyacrylamide gel electrophoresis.Methods] Pure RCG is applied to conduct phosphate buffer of polyacrylamide gel electrophoresis.Results] After being purified,RCG realizes seclusion of amylase.According to the electrophoretic mobility of polyacrylamide gel electrophoresis,RCG could be obviously identified.Conclusions] This method has good repeatability and could be used for the qualitative identification of RCG. |
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| Keywords: | Ray Cartilage Glycosaminoglycans Purification Polyacrylamide gel electrophoresis Identification |
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