Evaluation of selective media in antimicrobial surveillance programs capturing broad-spectrum β-lactamase producing Escherichia coli from chickens at slaughter |
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| Authors: | Kazal K Ghosh Nicol Janecko Agnes Agunos Anne Deckert Richard J Reid-Smith Sheryl Gow Joseph E Rubin |
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| Institution: | Department of Veterinary Microbiology (Ghosh, Rubin), Department of Large Animal Medicine (Gow), Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4; Canadian Integrated Program for Antimicrobial Resistance Surveillance, Centre for Foodborne Environmental Zoonotic Infectious Diseases, Public Health Agency of Canada, Laboratory for Foodborne Zoonoses, 160 Research Lane, Guelph, Ontario N1G 5B2 (Janecko, Agunos, Deckert, Reid-Smith) |
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| Abstract: | Antimicrobial resistance surveillance targeting agricultural animals is practiced in many countries but does not often include media selective for cephalosporin resistance. Here, we compared the frequency of recovery of resistant Escherichia coli using selective and non-selective media from the cecal contents of 116 chickens collected by the Canadian Integrated Program for Antimicrobial Resistance Surveillance (CIPARS). Third generation cephalosporin resistance was detected in 24 samples including 12, 10, and 2 on selective, non-selective, and both media, respectively. Isolates producing the CTX-M-1 ESBL were grown from 11 samples, 10 on selective medium only. Our results suggest that current surveillance approaches underestimate the true prevalence of resistance to critically important antimicrobials. |
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