| 超声波对菜豆种子超氧化物歧化酶活性的影响 |
| |
| 引用本文： | 钱春梅,谭兆赞,李云,巫光宏,黄卓烈.超声波对菜豆种子超氧化物歧化酶活性的影响[J].华南农业大学学报,2004,25(3):73-77. |
| |
| 作者姓名： | 钱春梅 谭兆赞 李云 巫光宏 黄卓烈 |
| |
| 作者单位： | 华南农业大学,生命科学学院,广东,广州,510642;华南农业大学,园艺学院,广东,广州,510642 |
| |
| 基金项目： | 国家自然科学基金资助项目(10074016) |
| |
| 摘 要： | 菜豆种子粗酶液经乙醇-氯仿、丙酮沉淀、DEAE-纤维素柱层析和Sephadex G-75柱层析，获得比活力为127．45U／mg的超氧化物歧化酶(SOD)，该酶活性受H2O2和KCN强烈抑制，经电泳鉴定为3种SOD同工酶，部分纯化的SOD证明为Cu，Zn-SOD，经不连续聚丙烯酰胺凝胶电泳获得其中1种电泳纯的酶，超声波处理此酶后，酶活力发生改变，适宜的超声波参数能显著提高酶活力，经超声波处理后，SOD的紫外吸收光谱无明显变化，而紫外差示光谱在一定波长范围内出现正峰和负峰。
|
| 关 键 词： | 菜豆 超氧化物歧化酶 超声波 |
| 文章编号： | 1001-411X(2004)03-0073-05 |
Effect of ultrasound on the activity of superoxide dismutase from kidney bean seeds |
| |
| QIAN Chun-mei,TAN Zhao-zan,LI Yun,WU Guang-hong,HUANG Zhuo-lie.Effect of ultrasound on the activity of superoxide dismutase from kidney bean seeds[J].Journal of South China Agricultural University,2004,25(3):73-77. |
| |
| Authors: | QIAN Chun-mei TAN Zhao-zan LI Yun WU Guang-hong HUANG Zhuo-lie |
| |
| Abstract: | Isoenzyme of superoxide dismutase(SOD) from kidney beans (Phaseolus vulgaris . L) seeds was purified by steps involving crude extract, chloroform-ethanol treatment, acetone precipitation, DEAE-cellulose chromatography and Sephadex G-75 chromatography. It had a specific activity of 127.45 U /mg. Its activity was strongly inhibited by H2O2 and KCN. It was proved that the purified enzyme was copper-and zinc-containing SOD. One of the isoenzymes was purified to homogeneity by discontinuous PAGE. The activity of this SOD could be changed by ultrasound treatment. The activity of SOD was increased after it was dealt with by ultrasound of suitable parameters. The ultraviolet absorption spectrum of SOD had no obvious change after treatment by ultrasound. Differential UV-spectra presented distinct positive peaks and negative peaks within certain wavelenth after ultrasound treatment. |
| |
| Keywords: | kidney bean superoxide dismutase ultrasound |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《华南农业大学学报》浏览原始摘要信息 |
| 点击此处可从《华南农业大学学报》下载免费的PDF全文 |
