| 多孢木霉HZ-31菌株胁迫下野燕麦内参基因的选择 |
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| 引用本文： | 朱海霞.多孢木霉HZ-31菌株胁迫下野燕麦内参基因的选择[J].草地学报,2021,29(8):1666-1674. |
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| 作者姓名： | 朱海霞 |
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| 作者单位： | 1. 青海大学农林科学院, 青海 西宁 810016;2. 农业部西宁作物有害生物科学观测实验站, 青海 西宁 810016;3. 青海省农业有害生物综合治理重点实验室, 青海 西宁 810016 |
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| 基金项目： | 青海省科技厅应用基础研究项目（2019-ZJ-7057）资助 |
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| 摘 要： | 为构建多孢木霉（Trichoderma polysporum） HZ-31菌株胁迫下野燕麦（Avena fatua L.）基因表达的RT-qPCR技术体系，筛选稳定内参基因，本研究利用GeNorm，NormFinder，BestKeeper和RefFinder 4个程序，对接种多孢木霉HZ-31菌株的野燕麦样本中8种候选内参基因（18S，28S，TUA，UBC，ACT，GAPDH，TBP和EF-1α）进行基于SYBR Green的RT-qPCR分析。结果表明TBP，18S和UBC是木霉侵染时野燕麦中表达最稳定的内参基因，TBP和TUA，TBP和GAPDH，18S和TBP，UBC和18S为最适宜两内参基因组合，可作为多孢木霉HZ-31菌株与野燕麦互作过程中功能基因表达分析的内参基因。
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| 关 键 词： | 野燕麦 内参基因 多孢木霉HZ-31菌株 表达稳定性 |
| 收稿时间： | 2020-08-28 |
Selection of Reference Genes in wild oats Avena fatua Induced by Trichoderma polysporum HZ-31 |
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| ZHU Hai-xia.Selection of Reference Genes in wild oats Avena fatua Induced by Trichoderma polysporum HZ-31[J].Acta Agrestia Sinica,2021,29(8):1666-1674. |
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| Authors: | ZHU Hai-xia |
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| Institution: | 1. Academy of Agriculture and Forestry Sciences of Qinghai University, Xining, Qinghai Province 810016, China;2. Scientific Observing and Experimental Station of Crop Pest in Xining in Ministry of Agriculture, Xining, Qinghai Province 810016, China;3. Key Laboratory of Agricultural Integrated Pest Management in Qinghai Province, Xining, Qinghai Province 810016, China |
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| Abstract: | In order to construct a RT-qPCR system suitable for stress of Trichoderma polysporum HZ-31 to wild oats (Avena fatua L.), and to screen stable internal reference genes, GeNorm, NormFinder, BestKeeper and RefFinder were used to analyze 8 candidates of internal reference genes (18S, 28S, TUA, UBC, ACT, GAPDH, TBP and EF-1α) in A. fatua after inoculation of T. polysporum HZ-31. The results showed that TBP, 18S and UBC were the most stable internal reference genes, TBP and TUA, TBP and GAPDH, 18S and TBP, UBC and 18S were the most suitable combination of the two internal reference genes, which could be used as the internal reference genes for functional gene expression analysis during the interaction between T. polysporum HZ-31 and A. fatua. |
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| Keywords: | Avena fatua L Internal reference gene Trichoderma polysporum HZ-31 Expression stability |
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