Pelargonium flower-break and pelargonium line pattern viruses in the Netherlands; purification,antiserum preparation,serological identification,and detection in pelargonium by ELISA |
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| Authors: | I Bouwen D Z Maat |
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| Institution: | (1) DLO-Research Institute for Plant Protection (IPO-DLO), P.O. Box 9060, 6700 GW Wageningen, the Netherlands |
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| Abstract: | Two viruses, detected frequently in the Netherlands in pelargonium, were identified by serology and test plant reactions. Antisera were prepared and an ELISA procedure was developed to detect the viruses in pelargonium.One of the viruses, PFBV-N, proved to be pelargonium flower-break virus. With the antiserum to PFBV-N, it could be detected reliably throughout the year inPelargonium zonale  Springtime Irene .The other virus, PLPV-N, was serologically closely related to pelargonium line pattern virus (PLPV) and to pelargonium ring pattern virus (PRPV), as were an old virus isolate from Saturnus, collected in the Netherlands in 1971 (L128), and PLPV isolates from Yugoslavia (PLPV-Y) and Denmark (PLPV-D). There were only minor differences in host-plant reactions between the virus isolates. Based on these tests, PLPV and PRPV are considered as isolates of the same virus, for which, for practical reasons, the name pelargonium line pattern virus is proposed.PLPV could be reliably detected by ELISA inP. zonale Springtime Irene and Amanda throughout the year with only a few exceptions. InPelargonium peltatum Tavira, however, reslts were erratic due to uneven distribution of virus in the plant. Best results were obtained when petioles of fully expanded leaves were tested. |
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| Keywords: | alfalfa mosaic virus arabis mosaic virus artichoke Italian latent virus cucumber mosaic virus indexing pelargonium leaf curl virus pelargonium ringspot virus pelargonium vein clearing virus pelargonium xonate spot virus tobacco mosaic virus tobacco necrosis virus tobacco rattle virus tobacco ringspot virus tomato black ring virus tomato ringspot virus tomato spotted wilt virus virus distribution |
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