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| 通过农杆菌介导法将口蹄疫结构蛋白全长基因P1导入大豆的研究 | |
| 引用本文： | 王萍,郭永来,王罡,季静,余云舟,李昌,金宁一.通过农杆菌介导法将口蹄疫结构蛋白全长基因P1导入大豆的研究[J].西南农业学报,2005,18(5):643-646. |
| 作者姓名： | 王萍 郭永来 王罡 季静 余云舟 李昌 金宁一 |
| 作者单位： | 1. 淮海工学院海洋学院,江苏,连云港,222005 2. 长春龙洋大豆生物蛋白股份有限公司,吉林,长春,130041 3. 天津大学农业与生物工程学院,天津,300072 4. 军事医学科学院军事兽医研究所,吉林,长春,130062 |
| 基金项目： | 吉林省科学技术厅资助项目（20030552-4） |
| 摘 要： | 本研究以大豆体细胞胚为受体。用农杆菌介导法将口蹄疫病毒结构蛋白全长基因P1导入大豆基因组中．获得了抗性植株。经GUS染色、PCR及PCR-southern杂交等分子检测。证明目的基因已导入并整合到大豆基因组中。为利用大豆作为生物反应器生产口蹄疫新型口服疫苗奠定了基础。 |
| 关 键 词： | 大豆 农杆菌介导 遗传转化 P1基因 |
| 文章编号： | 1001-4829（2005）05-0643-04 |
| 收稿时间： | 2005-07-16 |
| 修稿时间： | 2005-07-16 |
Transformation of structure protein gene P1 of foot and mouth disease virus into soybean via Agrobacterium-mediated |
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| WANG Ping,GUO Yong-lai,WANG Gang,JI Jing,YU Yun-zhou,LI Chang,JIN Ning-yi.Transformation of structure protein gene P1 of foot and mouth disease virus into soybean via Agrobacterium-mediated[J].Southwest China Journal of Agricultural Sciences,2005,18(5):643-646. | |
| Authors: | WANG Ping GUO Yong-lai WANG Gang JI Jing YU Yun-zhou LI Chang JIN Ning-yi |
| Abstract: | In this paper, structure protein gene P1 of foot and mouth disease virus was transformed into somatic embryos of soybean via Agrobacterium-mediated. The regenerated plantlets, which were resistant to kanamicy, were obtained and examined by PCR and PCRsouthern blot. The results indicated that target gene had been transformed into soybean. It could afford the basic research for soybean as bioreactor to produce oral vaccines against FMDV. |
| Keywords: | soybean Agrobacterium-mediated genetic transformation gene P1 |
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