Taxonomic characterization of Pyricularia isolates from green foxtail and giant foxtail, wild foxtails in Japan |
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| Authors: | Akiko Yamagashira Chihiro Iwai Masakazu Misaka Kenji Hirata Yoshikatsu Fujita Yukio Tosa Motoaki Kusaba |
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| Institution: | (1) Laboratory of Plant Pathology, Faculty of Agriculture, Saga University, Honjo1, Saga 840-8502, Japan;(2) Department of Medical Genetics, Majors of Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan;(3) National Agricultural Research Center, Rice Disease Resistance Research Team, Tsukuba, Ibaraki, Japan;(4) Laboratory of Plant Pathology, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan |
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| Abstract: | Twenty-eight Pyricularia isolates from two wild foxtails—green foxtail (Setaria viridis) and giant foxtail (S. faberii)—in Japan were taxonomically characterized by DNA analyses, mating tests, and pathogenicity assays. Although most of the
isolates failed to produce perithecia in mating tests with Magnaporthe oryzae, a diagnostic polymerase chain reaction-restriction fragment length polymorphism phenotype of M. oryzae was detected in the beta-tubulin genomic region in all isolates. The pathogenicity assays revealed that host ranges of the
isolates were similar to those of isolates from foxtail millet (S.
italica), which were exclusively pathogenic on foxtail millet. In addition to the 28 isolates from wild foxtails, 22 Pyricularia isolates from 11 other grasses were analyzed by RFLP using single-copy sequences as probes. In a dendrogram constructed from
the RFLP data, isolates that were previously identified as M. oryzae formed a single cluster. All the wild foxtail isolates formed a subcluster with foxtail millet isolates within the M. oryzae cluster. From these results, we conclude that Pyricularia isolates from the wild foxtails are closely related to isolates from foxtail millet and should be classified into the Setaria pathotype of M. oryzae. |
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| Keywords: | Setaria viridis Setaria faberii Magnaporthe oryzae |
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