| 粗山羊草（Ae.tauschii）幼苗和根全长cDNA文库构建及其EST注释与比较分析 |
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| 引用本文： | 赵光耀,孔秀英,贾继增.粗山羊草（Ae.tauschii）幼苗和根全长cDNA文库构建及其EST注释与比较分析[J].中国农业科学,2007,40(7):1331-1336. |
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| 作者姓名： | 赵光耀 孔秀英 贾继增 |
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| 作者单位： | 1. 中国农业科学院作物科学研究所/农业部作物种质资源与生物技术重点开放实验室
2. 国家农作物基因资源与基因改良重大科学工程,北京,100081 |
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| 基金项目： | 引进国际先进农业科技计划(948计划);国家重点基础研究发展计划(973计划) |
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| 摘 要： | 【目的】构建小麦D基因组供体粗山羊草根和叶的全长cDNA文库，探讨利用生物信息学方法分析发掘组织特异性表达基因的可行性。【方法】本研究利用Cap-trapper方法构建全长cDNA文库，利用高通量测序技术获得大批高质量EST序列，在基于Linux系统的本地化生物信息学分析平台上对上述EST进行了电子注释分析。【结果】成功构建了粗山羊草根和叶的全长cDNA文库，测序获得根EST序列6 973条和叶EST 6 616条。利用本地化数据分析平台对其进行功能注释。利用GO-Diff软件，发现了两个文库间表达基因的功能差异，找到组织间表达差异显著的基因和组织内特异性表达的基因。【结论】利用构建的全长cDNA测序获得大量EST序列，通过生物信息学方法挖掘差异表达或特异性表达基因的方法是可行的。
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| 关 键 词： | 粗山羊草 表达序列标签 全长cDNA文库 功能注释 生物信息学 |
| 收稿时间： | 2006-5-24 |
| 修稿时间： | 2006-05-242006-09-30 |
Full-length cDNA Libraries Construction from Ae. tauschii Root and Shoot and EST Annotation and Comparative Analysis |
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| ZHAO Guang-yao,KONG Xiu-ying,JIA Ji-zeng.Full-length cDNA Libraries Construction from Ae. tauschii Root and Shoot and EST Annotation and Comparative Analysis[J].Scientia Agricultura Sinica,2007,40(7):1331-1336. |
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| Authors: | ZHAO Guang-yao KONG Xiu-ying JIA Ji-zeng |
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| Abstract: | Abstract: Expressed sequenced tags (EST) and full-length cDNA were important tool and resources for biological functional genomic and genetic research., the progenitor for D genome of bread wheat, was an important material for wheat genetic and genomic research.. Two full-length cDNA libraries were constructed from shoot and root of Aegilops tauschii Y2282 respectively by use of Cap-trapper method and 6616 ESTs and 6973 ESTs were obtained from the 2 libraries respectively. By use of our bioinformatic analysis platform base on Linux system ， we did electronic annotation for the EST, including blasting against public database such as protein ,nucleotide and triticum and Aegilops EST databases from NCBI. By use of result of GO ontology catalogue ,which was obtained from software GoPipee, and expression profile ,which were come from Phrap assembling result, some EST differently or specifically expressed in 2 cDNA libraries were obtained. Maybe the results could help us understand the molecular basis of different functions which organs perform. |
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| Keywords: | Ae tauschii EST Full-length cDNA library Function annotation Bioinformatics |
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