| 高产乳酸链球菌素生产菌的诱变 |
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| 引用本文： | 徐文生,陈湘宁,张艳艳,张大革,刘一倩.高产乳酸链球菌素生产菌的诱变[J].北京农学院学报,2010,25(1):37-40. |
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| 作者姓名： | 徐文生 陈湘宁 张艳艳 张大革 刘一倩 |
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| 作者单位： | 北京农学院,食品科学系,北京,102206;北京农学院,食品科学系,北京,102206;北京农学院,食品科学系,北京,102206;北京农学院,食品科学系,北京,102206;北京农学院,食品科学系,北京,102206 |
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| 基金项目： | 北京市教委面上课题资助“双歧杆菌群体感应基因baiS功能研究”(KM200910020011) |
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| 摘 要： | 为了获得高效价的Nisin生产菌株,采用传统的微生物育种技术,以Nisin生产菌乳酸乳球菌作为出发株,通过紫外照射和亚硝酸处理进行协同诱变育种。根据致死率和诱变剂量的相互关系,分别得出了紫外线照射和亚硝酸诱变的致死率曲线以选择合适的诱变剂量。最终确定采用2.5 min紫外线照射和4 min亚硝酸处理对乳酸乳球菌进行诱变获得突变菌株。突变株经初筛和复筛得到Nisin高产菌株,其发酵液Nisin的效价较出发株高1.72倍。
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| 关 键 词： | 诱变 乳酸乳球菌 致死率 乳酸链球菌素 |
Screening and breeding of high Nisin-producing mutant with UV and nitrous acid treatment |
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| XU Wen-sheng,CHEN Xiang-ning,ZHANG Yan-yan,ZHANG Da-ge,LIU Yi-qian.Screening and breeding of high Nisin-producing mutant with UV and nitrous acid treatment[J].Journal of Beijing Agricultural College,2010,25(1):37-40. |
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| Authors: | XU Wen-sheng CHEN Xiang-ning ZHANG Yan-yan ZHANG Da-ge LIU Yi-qian |
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| Institution: | Department of Food Science and Engineering;Beijing University of Agriculturel;Beijing 102206;China |
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| Abstract: | The traditional microbiological breeding technology was used to obtain a high Nisin-producing Lactococcus lactisstrain.The parental Nisin-producing strain was treated with UV and nitrous acid.According to the correlation of lethal rate and inducing dose,the lethality curve of UV and nitrous acid treatment were obtained in L.lactis,and the optimum treatment dose was determined.The mutant strain was obtained by induced breeding of parental strain after being treated with 2.5 min UV and 4 min nitrous acid.Afte... |
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| Keywords: | mutant Lactococcus lactis lethal rate nisin |
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