| 鸭肠炎病毒US2基因的序列测定与分析 |
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| 引用本文： | 李子剑,李云龙,陈苏,艾武,宋敏训,李玉峰.鸭肠炎病毒US2基因的序列测定与分析[J].山东农业科学,2008(3):33-36. |
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| 作者姓名： | 李子剑 李云龙 陈苏 艾武 宋敏训 李玉峰 |
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| 作者单位： | 1. 山东师范大学生命科学学院,山东,济南,250014
2. 山东省农业科学院家禽研究所,山东,济南,250023 |
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| 摘 要： | 以鸭肠炎病毒(DEV)SD-01株DNA为模板,根据GenBank上已发表的基因序列设计一对引物,利用PCR技术扩增出US2全基因,将目的条带连入pGM-T载体,得到的阳性重组质粒命名为pGM-US2并进行序列测定。将测序结果与疫苗株、鸭瘟鸡胚化弱毒疫苗(C-KCE)株和单纯疱疹病毒-1型(HSV-1)、马立克氏病病毒(MDV)、伪狂犬病病毒(PRV)的US2基因同源性进行比较,发现核苷酸和氨基酸的序列同源性分别为100%~25.8%和100%~28%。结果表明,US2基因在DEV中是完全保守的,但与其它疱疹病毒相比同源性较低。
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| 关 键 词： | 鸭肠炎病毒 US2基因 PCR 疱疹病毒 |
| 文章编号： | 1001-4942(2008)03-0033-04 |
| 修稿时间： | 2007年11月12 |
Sequencing and Analysis of US2 Gene of Duck Enteritis Virus |
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| LI Zi-jian,LI Yun-long,CHEN Su,AI Wu,SONG Min-xun,LI Yu-feng.Sequencing and Analysis of US2 Gene of Duck Enteritis Virus[J].Shandong Agricultural Sciences,2008(3):33-36. |
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| Authors: | LI Zi-jian LI Yun-long CHEN Su AI Wu SONG Min-xun LI Yu-feng |
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| Abstract: | The genomic DNA of DEV SD-01 strain was extracted and a pair of primers was designed according to the published nucleotide sequence in GenBank.The US2 gene was amplified by polymerase chain reaction(PCR) with extracted total DNA from DEV SD-01 strain as template and cloned into vector pGM-T.The positive recombinant plasmid was named pGM-US2 and sequenced.The results showed that the homology of the nucleotides and amino acid of US2 gene from DEV SD-01 strain with that of DEV vaccine strain,C-CKE strain,HSV-1,MDV and PRV were 100%~25.8% and 100%~28% respectively.These data suggested that US2 gene was completely conserved among different DEV strains,whereas showing low level homology with other herpesviruses. |
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| Keywords: | Duck enteritis virus US2 gene PCR Herpesvirus |
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