Rescue In Vitro Fertilization Method for Legacy
Stock of Frozen Mouse Sperm |
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| Authors: | Naomi NAKAGATA Toru TAKEO Kiyoko FUKUMOTO Yukie HARUGUCHI Tomoko KONDO Yumi TAKESHITA Yuko NAKAMUTA Tomoko UMENO Shuuji TSUCHIYAMA |
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| Institution: | 1)Division of Reproductive Engineering, Center for Animal Resources and Development (CARD), Kumamoto 860-0811, Japan |
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| Abstract: | Sperm cryopreservation has been widely adopted for maintenance of the genetically
engineered mouse (GEM). The cryopreserved sperm are being exchanged among many institutes
worldwide. However, the recipients are not always able to obtain high fertilization rates
with the frozen sperm shipped from senders. In this study, we cryopreserved mouse sperm
via various methods and performed in vitro
fertilization (IVF) in which the combination of methyl-beta-cyclodextrin for sperm
preincubation and reduced glutathione for insemination was used (the MBCD-GSH IVF). In
addition, frozen sperm sent from the Jackson Laboratory (USA) were thawed and used for IVF
in the same manner. The fertilization rates of both the sperm cryopreserved
via the methods applied in some countries and the cryopreserved GEM
sperm improved when used with the MBCD-GSH IVF method. Therefore, we strongly believe that
the MBCD-GSH IVF method brings about relatively high fertilization rates with any strain
of frozen mouse sperm. |
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| Keywords: | C57BL/6 Cryopreserved sperm In vitro fertilization Mouse Transport |
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