| 猪乳中一组高分子量多态性蛋白质的同源性鉴定 |
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| 引用本文： | 张海方,邹思湘,秦宜德,陈伟华.猪乳中一组高分子量多态性蛋白质的同源性鉴定[J].中国农业科学,2004,37(9):1369-1373. |
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| 作者姓名： | 张海方 邹思湘 秦宜德 陈伟华 |
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| 作者单位： | 南京农业大学农业部动物生理生化重点开放实验室,南京,210095 |
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| 基金项目： | 国家自然科学基金资助项目(39770554) |
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| 摘 要： | 利用硫酸铵沉淀、凝胶过滤层析和阴离子交换层析等方法从猪乳中分离纯化高分子量蛋白质B (HMWP-B)。 制备兔抗HMWP-B的多克隆抗体，免疫印迹结果表明，不同带型的HMWP之间存在免疫交叉反应，说明该组蛋白具有同源性，为其多态性假说提供了证据。
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| 关 键 词： | 猪乳 高分子量蛋白质 多态性 同源性 |
| 收稿时间： | 2003-11-20 |
Homogenous Identification of a Group of Polymorphic High Molecular Weight Protein in Sow Milk |
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| ZHANG Hai-fang,ZOU Si-xiang,QIN Yi-de,CHEN Wei-hua.Homogenous Identification of a Group of Polymorphic High Molecular Weight Protein in Sow Milk[J].Scientia Agricultura Sinica,2004,37(9):1369-1373. |
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| Authors: | ZHANG Hai-fang ZOU Si-xiang QIN Yi-de CHEN Wei-hua |
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| Abstract: | High molecular weight proteins (HMWP) in sow milk showed four variants of A, B, C, and D by SDS-PAGE. HMWP-B was purified by a combination of ammonium precipitation, SephadexG-200 gel filtration chromatography and High Q column anion-exchange chromatography. The rabbit anti- HMWP-B Polyclonal Antibody was then raised. Immunoblotting of HMWP in sow milk showed immunological cross reaction among these variants and indicated HMWP in sow milk are homologous. The result of this study provides a proof for the hypothesis of polymorphism of HMWP . |
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| Keywords: | Sow milk High molecular weight protein (HMWP) Polymorphism Homology |
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