Effect of β‐glucooligosaccharides as a new prebiotic for dietary supplementation in olive flounder (Paralichthys olivaceus) aquaculture |
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| Authors: | Md Tawheed Hasan Won Je Jang Seunghan Lee Kang Woong Kim Bong‐Joo Lee Hyon‐Sob Han Sungchul C Bai In‐Soo Kong |
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| Institution: | 1. Department of Biotechnology, Pukyong National University, Busan, Republic of Korea;2. Department of Marine Bio‐Materials and Aquaculture, Pukyong National University, Busan, Republic of Korea;3. Aquafeed Research Center, NIFS, Pohang, Republic of Korea |
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| Abstract: | β‐Glucooligosaccharides (BGO), produced from barley β‐glucan, were used as a feed supplement (0.1%) for juvenile olive flounder (Paralichthys olivaceus) to identify and quantify its oral administration effects on innate immunomodulation and infectious disease protection. Juvenile flounders (14 ± 0.5 g) were divided into two groups fed either 0.1% BGO (treatment) or a standard diet (control) for 8 weeks. At the end of the experiment, investigation of the effects was carried out through systemic studies on growth performance, serum and mucus biochemical parameters, innate immunity, microvillus length, and relative pro‐inflammatory cytokine gene expression. The results demonstrated that the BGO diet produced slightly higher levels of growth performance, serum protein, microvillus length and pro‐inflammatory cytokine gene (tumour necrosis factor‐α, interleukin IL]‐1β, and IL‐6) expression without any significant differences (p > .05). All innate immunity parameters were up‐regulated by BGO administration and, among these, respiratory burst, lysozyme and superoxide dismutase (SOD) were significantly different (p < .05). Fish in the both groups were challenged with Streptococcus iniae (1.35 × 108 CFU/ml), and BGO group was focused to confirm the promotion of innate immunity parameters. The results showed a significantly (p < .05) lower death rate compared with that of the control. Therefore, BGO could be used as a new prebiotic in future olive flounder aquaculture as well as to control streptococcosis. |
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| Keywords: | β ‐glucooligosaccharides growth performance innate immune response olive flounder pro‐inflammatory cytokine |
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