| 吖啶橙简易免疫荧光术用于快速检出鸡白痢和禽伤寒沙门氏菌的方法研究 |
| |
| 引用本文： | 文心田,夏定友,廖德惠,谢镜怀.吖啶橙简易免疫荧光术用于快速检出鸡白痢和禽伤寒沙门氏菌的方法研究[J].四川农业大学学报,1986(2). |
| |
| 作者姓名： | 文心田 夏定友 廖德惠 谢镜怀 |
| |
| 作者单位： | 四川农业大学兽医学系，四川农业大学兽医学系，四川农业大学兽医学系，四川农业大学兽医学系 |
| |
| 摘 要： | 本文提出一种快速检出S.p.和S.g.的简易方法——吖啶橙免疫荧光菌团培养浓集法(AOIFBC法)。此法将吖啶橙高倍稀释后与少量沙门氏菌0:9单因子血清混合,配制成吖啶橙荧光抗体(AOFA),然后取少量加入经4小时增菌的液体标本中,再孵育1—4小时,可使与抗体结合的S.p.或S.g.凝集繁殖成亮绿色荧光菌团,低速离心沉淀后,在低倍荧光镜下容易检出。荧光菌团可取出培养进一步验证。试验表明,此法敏感度比无吖啶橙的显微沉淀凝集试验和玻片凝集试验分别高100倍和2500—5000倍。作AOIFBC法检查,特异性强:与鼠伤寒沙门氏菌等七种常见细菌无交叉荧光菌团形成;敏感度高:每毫升含S.p.活菌84×10~2个和S.g.活菌76×10~1个以上,可呈“++”→“++++”荧光菌团。本法对雏鸡粪液模拟标本检测,获得肯定的阳性结果。
|
| 关 键 词： | AVIAN DISFAE(49NB)禽病(Qinbing) A CRIDINE(34G)吖啶(Yading) IMMUNO FLUORESCENCE METHOD(32EH 34BB) 免疫荧光法(Mianyi Yingguangfa) SALMONELLA PULLORUM鸡白痢沙门氏菌(Ji Baili Shamenshijun) SALMONFLLA GALLINARUM禽伤寒沙门氏菌(Qin Shanghan Shamenshijun) RAPID DIAGNOSIS快速诊断(Kuaisu Zhenduan) |
STUDIES ON RAPID DIAGNOSIS OF SALMONELLA PULLORUM-GALLINARUM BY CULTURING AND CENTRIFUGALIZING ACRIDINE ORANGE IMMUNOFLUORESCENCE CLUMPS |
| |
| Wen Xintian et al.STUDIES ON RAPID DIAGNOSIS OF SALMONELLA PULLORUM-GALLINARUM BY CULTURING AND CENTRIFUGALIZING ACRIDINE ORANGE IMMUNOFLUORESCENCE CLUMPS[J].Journal of Sichuan Agricultural University,1986(2). |
| |
| Authors: | Wen Xintian |
| |
| Abstract: | In this paper a simple method was put forward for rapid diagnosis of Salmonella pullorum gallitiarum by culturing and centrifugalinzine orange immunofluorescence clumps. The bacteria cultured for four hours in medium, when stained mith a mixed scolution of highly diluted specific antiserum and acridine orange (AOFA), could be mads to form specific brilliant green fluorascenct clumps in 1 4 hours. Then the viable bacteria may be isolated by immunofluorescent clumps and incubated for identification. AOFA of S. pullorum gall inarum was specific in the forming of fluorescent clumps, which Could not be rendered by tests carried out with Salmonella typhimurum and other bacteria of 7 species. The method gave a high sensitivity to the samples with S. pullorum number over 84×12~2/ml and S. gallinarum number over 76×10~1/ml. Astrong positive reaction (**→++++) of immunofluorescent bacterial clumps existed. |
| |
| Keywords: | AVIAN DISFAE(49NB)(Qinbing) ACRIDINE(34G) (Yading) IMMUNO FLUORESCENCE METHOD (32EH 34BB) (Mianyi Yingguangfa) SALMONELLA PULLORUM(Ji Baili Shamenshijun) SALMONFLLA GALLINARUM (Qin Shanghan Shamenshijun) RAPID DIAGNOSIS(Kuaisu Zhenduan) |
| 本文献已被 CNKI 等数据库收录！ |
