| 以棉花茎尖分生组织为受体进行基因枪轰击转化的研究 |
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| 引用本文： | 王省芬,迟吉娜,马峙英.以棉花茎尖分生组织为受体进行基因枪轰击转化的研究[J].棉花学报,2006,18(1):53-57. |
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| 作者姓名： | 王省芬 迟吉娜 马峙英 |
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| 作者单位： | 河北农业大学,河北省作物种质资源重点实验室,河北,保定,071001 |
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| 基金项目： | 河北省科技厅博士基金;河北农业大学校科研和教改项目 |
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| 摘 要： | 以棉花茎尖分生组织为受体进行基因枪遗传转化,在不受宿主基因型范围和操作时间的限制等方面优于农杆菌介导法和花粉管通道技术。影响棉花茎尖分生组织基因枪遗传转化率的因素较为复杂,轰击前,外植体制备需考虑茎尖分生组织的取材时间、下胚轴保留的长度、外源生长调节剂及活性炭的使用量,以保证其正常生长;轰击后,抗生素筛选的浓度、筛选培养基的转换时间和抗性苗的壮苗也会影响转化效率;此外,DNA和钨粉或金粉的质量、基因枪参数及操作等对转化效果都有一定的作用。除对影响茎尖分生组织基因枪转化率的因素分析以外,还对近年来在棉花茎尖分生组织遗传转化中的最新动态进行了综述,并对目前存在的问题加以讨论。
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| 关 键 词： | 棉花 基因枪轰击 遗传转化 茎尖分生组织 植株再生 |
| 文章编号： | 1002-7807(2006)01-0053-05 |
| 修稿时间： | 2005年9月21日 |
Advances on Genetic Transformation of Cotton Shoot Apical Meristem via Gene-gun Bombardment |
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| WANG Xing-fen,CHI Ji-na,MA Zhi-ying.Advances on Genetic Transformation of Cotton Shoot Apical Meristem via Gene-gun Bombardment[J].Cotton Science,2006,18(1):53-57. |
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| Authors: | WANG Xing-fen CHI Ji-na MA Zhi-ying |
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| Abstract: | Genetic transformation of cotton shoot apical meristem via gene-gun bombardment is advantageous over Agrobacterium-meditated transformation and pollen-tube pathway in the genotype-independent and time-consuming. There are many factors affecting the shoot-apex transformation. Before shooting, preparation of explants is important for normal growth. The time of cuttin apical meristem, the remained length of hypocotyls, the usage content of exogenous hormones or active carbon must be considered. After shooting, the selected concentration of antibiotics, the period of media transition and seedling rejuvenated influenced the efficiency of shoot-apex method. Moreover, some physical factors were also essential to transformation, such as the quality of DNA and tungsten or gold powder, bonding of DNA, the parameters and manipulations of particle gun. Some factors were analyzed affecting the transformation efficiency of cotton shoot apical meristem via gene-gun bombardment, and the latest development in cotton genetic transformation of the shoot apical meristem were reviewed. In the paper ,The existing problems on the method of transformation were also discussed. |
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| Keywords: | cotton particle bombardment genetic transformation shoot apical meristem plant regeneration |
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