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| 冬樱花愈伤组织诱导和抑制褐化初探 | |
| 引用本文： | 和凤美,朱永平,杨晓红,李璇,邵琬珊.冬樱花愈伤组织诱导和抑制褐化初探[J].中国农学通报,2010,26(12):130-134. |
| 作者姓名： | 和凤美 朱永平 杨晓红 李璇 邵琬珊 |
| 作者单位： | 1. 云南农业大学园林园艺学院,昆明,650201 2. 云南农业大学农学与生物技术学院,昆明,650201 3. 昆明学院生命科学与技术系,昆明,650031 |
| 摘 要： | 以冬樱花叶片、花托、花梗、子房和苞片为外植体，在附加不同浓度的生长素NAA和细胞分裂素6-BA组合的MS培养基上进行愈伤组织诱导并探讨愈伤组织褐化的抑制，实验结果表明：在供试的培养基中，叶片的愈伤组织诱导率最高，花器官愈伤组织诱导时，花梗诱导率最高，其次为花托、子房，而苞片诱导率为0。缩短外植体消毒时间、遮光及加入活性炭，可有效降低愈伤组织的褐化。 |
| 关 键 词： | 原生质体 原生质体 制备 应用 |
| 收稿时间： | 2010-02-01 |
| 修稿时间： | 2010-02-28 |
The Primary Investigation on Callus Induction and Browning Prevention of Cerasus Cerassoides |
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| He Fengmei,Zhu Yongping,Yang Xiaohong,Li Xuan,Shao Wanshan.The Primary Investigation on Callus Induction and Browning Prevention of Cerasus Cerassoides[J].Chinese Agricultural Science Bulletin,2010,26(12):130-134. | |
| Authors: | He Fengmei Zhu Yongping Yang Xiaohong Li Xuan Shao Wanshan |
| Institution: | (College of Horticulture and Landscape, Yunnan Agricultural University, Kunming 650201; 2College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming 650201; College of life Science and Technology, Kunming University, Kunming 650031) |
| Abstract: | In this paper, we studied induction of callus and browning prevention on MS addition with different concentration of 6-BA and NAA using receptacle, peduncle, ovary and leaf blade of cerasus cerassoides as explants. The results showed that the callus induction rate of leaf was the highest in all tested culture media and explants, and among callus induction of floral organogenesis, peduncle was the best, followed by receptacle and ovary, and that of bracteolate was the lowest. Browning of callus can be effectively decreased with shorting explants disinfection time, keeping away from light, addition with active carbon. |
| Keywords: | tissue culturezz |
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