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| 动物源大肠杆菌及沙门氏菌抗药性的快速检测 | |
| 引用本文： | 朱小玲,杜加法,蔡亚娜,胡明,白华,齐静,刘玉庆.动物源大肠杆菌及沙门氏菌抗药性的快速检测[J].中国动物检疫,2010,27(6):40-42. |
| 作者姓名： | 朱小玲 杜加法 蔡亚娜 胡明 白华 齐静 刘玉庆 |
| 作者单位： | 1. 山东农业科学院畜牧兽医研究所,山东济南,250100 2. 山东农业科学院畜牧兽医研究所,山东济南,250100;湖南农业大学动物医学院,湖南长沙,410128 3. 山东农业科学院畜牧兽医研究所,山东济南,250100;青岛农业大学动物医学院,山东青岛,266109 |
| 基金项目： | 抗生素残留引起细菌耐药性安全控制技术的研究与示范，细菌群体在抗生素作用下形成的微菌落与抗药性突变发生之关系的研究，山东省自然科学基金资助项目 |
| 摘 要： | 利用本室自主研制的新型96点阵药敏检测盒检测山东地区动物源大肠杆菌、沙门氏菌的药物敏感性,试验结果表明,大肠杆菌对氨苄西林和喹诺酮类药物的抗药率达98%以上,禽源大肠杆菌的抗药率高于猪源大肠杆菌,猪源大肠杆菌的抗药谱广于禽源大肠杆菌;沙门氏菌抗药率略低于大肠杆菌,但同样呈现多重抗药,主要集中在5抗-7抗和13抗-14抗两个范围。96点阵抗生素药敏检测盒是适合于试验室进行大规模抗药性监测的新工具,具有简易、准确、快速、大通量、节约的优点,为临床治疗中抗生素的使用、细菌病的治疗提供了技术保障,从而达到避免盲目使用抗生素,降低细菌抗药率的目的。 |
| 关 键 词： | 96点阵药敏检测盒 大肠杆菌 沙门氏菌 喹诺酮类 多重抗药性 |
Rapid Detection of Drug Resistance of Escherichia coil and Salmonella Isolates from Animals |
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| Zhu Xiaoling,Du Jiaf,Cai Yan,Hu Ming,Bai hu,Qi Jing,Liu Yuqing.Rapid Detection of Drug Resistance of Escherichia coil and Salmonella Isolates from Animals[J].China Journal Of Animal Quarantine,2010,27(6):40-42. | |
| Authors: | Zhu Xiaoling Du Jiaf Cai Yan Hu Ming Bai hu Qi Jing Liu Yuqing |
| Institution: | 1.Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences,Jinan 250100,China;2.Veterinary Faculty Hunan Agricultural University,Changsha 410128,China;3.Veterinary Faculty,Qingdao Agricultural University,Qingdao 266109,China) |
| Abstract: | The drug susceptibility of Escherichia coli and Salmonella from poultry and swine in Shandong was detected by new type 96 dot-matrix antibiotic susceptibility testing kit.Experimental results showed that the resistance rates of Escherichia coli to AMP and QNS reached over 98%,the resistance rate of Escherichia coli from poultry was higher than from swine and the resistance spectrum of Escherichia coli from swine was wider than from poultry.The resistance rate of Salmonella was lower than Escherichia coli,but presented multiple resistanta-nce.The 96 dot-matrix antibiotic susceptibility testing kit was simple,exact,fast,large-flux and frugal and was very suitable to large-scale drug resistance monitoring in the laboratory.The new tool provided technical support for antibiotics application and the treatment of bacterial disease,thus avoiding blind use of antibiotics and reducing the drug resistancerate. |
| Keywords: | 96 dot-matrix antibiotic susceptibility testing kit Escherichia coli(E coli)i Salmonella Quinolones(QNS) Multi-drug resistance |
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