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| 抗生素对高羊茅胚性愈伤组织生长与分化的影响 | |
| 引用本文： | 吴关庭,胡张华,郎春秀,陈笑芸,金卫,王伏林,陈锦清,夏英武.抗生素对高羊茅胚性愈伤组织生长与分化的影响[J].核农学报,2005,19(2):88-91. |
| 作者姓名： | 吴关庭 胡张华 郎春秀 陈笑芸 金卫 王伏林 陈锦清 夏英武 |
| 作者单位： | 1. 浙江大学原子核农业科学研究所,浙江,杭州,310029;浙江省农业科学院病毒学与生物技术研究所,浙江,杭州,310021 2. 浙江省农业科学院病毒学与生物技术研究所,浙江,杭州,310021 3. 浙江大学原子核农业科学研究所,浙江,杭州,310029 |
| 摘 要： | 研究了两种常用抗生素对高羊茅胚性愈伤组织生长和分化的影响 ,结果表明 ,头孢霉素明显抑制愈伤组织生长 ,而浓度低于 5 0 0mg L的羧苄青霉素能促进愈伤组织生长 ;两种抗生素对愈伤组织分化均有抑制作用 ,但羧苄青霉素的抑制作用要比头孢霉素小。因此 ,在农杆菌介导高羊茅遗传转化中 ,抑菌剂以选用羧苄青霉素较为合适。 |
| 关 键 词： | 高羊茅 胚性愈伤组织 生长 分化 抗生素 |
| 文章编号： | 1000-8551(2005)02-088-04 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2004年10月11 |
THE EFFECTS OF ANTIBIOTICS ON GROWTH AND DIFFERENTIATION OF EMBRYOGENIC CALLI OF TALL FESCUE |
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| WU Guan-Ting,HU Zhang-Hua,LANG Chun-Xiu,CHEN Xiao-yun,JIN Wei,WANG Fu-lin,CHEN Jin-qing,XIA Ying-wu.THE EFFECTS OF ANTIBIOTICS ON GROWTH AND DIFFERENTIATION OF EMBRYOGENIC CALLI OF TALL FESCUE[J].Acta Agriculturae Nucleatae Sinica,2005,19(2):88-91. | |
| Authors: | WU Guan-Ting HU Zhang-Hua LANG Chun-Xiu CHEN Xiao-yun JIN Wei WANG Fu-lin CHEN Jin-qing XIA Ying-wu |
| Institution: | WU Guan-ting 1 HU Zhang-hua 2 LANG Chun-xiu 2 CHEN Xiao-yun 2 JIN Wei 2 WANG Fu-lin 2 CHEN Jin-qing 2 XIA Ying-wu 1 |
| Abstract: | The effects of two widely used antibiotics on growth and differentiation of embryogenic calli of tall fescue were investigated. The results showed that callus growth was obviously suppressed by cefotaxime, but stimulated by carbenicillin at the concentration less than 500mg/L. Callus differentiation was inhibited by both the antibiotics with greater inhibition produced by cefotaxime. Therefore, carbenicillin was the preferable choice of the two tested antibiotics for Agrobacterium tumefaciens-mediated transformation of tall fescue. |
| Keywords: | tall fescue (Festuca arundinacea Schreb ) embryogenic callus growth differentiation antibiotics |
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