| 不同消毒处理对竹芋组培无菌外植体获得的影响 |
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| 引用本文： | 夏时云,付任胜,张德岚,马武赟.不同消毒处理对竹芋组培无菌外植体获得的影响[J].天津农业科学,2009,15(2):6-8. |
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| 作者姓名： | 夏时云 付任胜 张德岚 马武赟 |
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| 作者单位： | 1. 天津市农业生物技术研究中心,天津,300192
2. 天津农学院,天津,300384 |
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| 基金项目： | 天津市科委重点科技攻关专项项目 |
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| 摘 要： | 以过渡栽培植株的侧芽或顶芽为外植体,研究了不同消毒处理对竹芋组培无菌外植体获得的影响。结果表明:采用抗菌素浸泡5h、熏蒸12min并配合常规消毒处理(75%酒精浸泡1min;3.0%～5.0%的次氯酸钠和0.1%升汞1∶1混合溶液浸泡10～12min)对外植体进行综合灭菌,能较快地杀灭竹芋外植体表面细菌,使初次接种污染率控制在20%以下。
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| 关 键 词： | 竹芋 组织培养 消毒处理 污染率 外植体 |
Effect of Different Disinfection Treatment on Sterile Explant Obtaining in Tissue Culture of Calathea spp |
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| XIA Shi-yun,FU Ren-sheng,ZHANG De-lan,MA Wu-yun.Effect of Different Disinfection Treatment on Sterile Explant Obtaining in Tissue Culture of Calathea spp[J].Tianjin Agricultural Sciences,2009,15(2):6-8. |
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| Authors: | XIA Shi-yun FU Ren-sheng ZHANG De-lan MA Wu-yun |
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| Institution: | XIA Shi-yun, FU Ren-sheng, ZHANG De-lan, MA Wu-yun(1.Tianjin Research Center of Agricultural Biotechnology, Tianjin 300192, China ; 2.Tianjin Agricultural College, Tianjin 300384, China) |
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| Abstract: | The effect of different disinfection methods on explant obtaining in Calathea spp was studied. The results showed that pre-treatment and antibiotic marinating for 5 h and suffocating for 12 min as well as ordinary disinfection for 12 min for surface disinfection could effectively control explant contamination. The contaminate rate with this method would be less than 20%. |
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| Keywords: | Calathea spp tissue culture disinfection treatment contaminate rate explant |
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