| 秋水仙素诱导野生杜鹃花变异试验 |
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| 引用本文： | 杨华,兰红星,宋绪忠,杜国坚,陈卓梅.秋水仙素诱导野生杜鹃花变异试验[J].浙江林业科技,2009,29(6). |
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| 作者姓名： | 杨华 兰红星 宋绪忠 杜国坚 陈卓梅 |
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| 作者单位： | 1. 浙江省林业科学研究院,浙江,杭州,310023
2. 浙江省松阳县林业技术推广站,浙江,松阳,323400 |
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| 摘 要： | 应用0.5、1.0、2.0、3.0 mg/mL 4种浓度的秋水仙素溶液诱导正在发育的映山红(Rhododendron simsii)、马银花(Rh.ovatum)、满山红(Rh.mariesii)的花芽和叶芽,观察其花粉粒及气孔变异情况.结果表明,3种杜鹃叶芽和花芽表现出的多倍性有一定的变化,映山红花粉粒在3.0 mg/mL的浓度处理时变异最大,增加37.99%;满山红花粉粒在2.0 mg/mL的浓度处理时变异最大,增加21.96%;随着秋水仙素浓度的增加,3种杜鹃的气孔器大小变异增加,在3.0 mg/mL浓度处理时增加最多,出现气孔增长、保卫细胞宽增加、保卫细胞长度增加等多倍体表现;气孔密度变化与多倍性联系规律不明显.
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| 关 键 词： | 秋水仙素 满山红 映山红 马银花 杜鹃 花粉 气孔 |
Experiment on Induction of Three Species of Rhododendron by Colchicines |
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| YANG Hua,LAN Hong-xing,SONG Xu-zhong,DU Guo-jian,CHEN Zhuo-mei.Experiment on Induction of Three Species of Rhododendron by Colchicines[J].Journal of Zhejiang Forestry Science and Technology,2009,29(6). |
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| Authors: | YANG Hua LAN Hong-xing SONG Xu-zhong DU Guo-jian CHEN Zhuo-mei |
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| Abstract: | Induction was conducted on developing flower and leaf bud of Rhododendron simsii, R. ovatum and K mariesii with four treatments of colchicines concentrations, 0.5mg/mL, 1.0 mg/mL, 2.0 mg/mL and 3.0 mg/mL. Observation on microspore and stomata of tested leaf and flower buds demonstrated polyploid variation. Microspore of K simsii increased 37.99% with 3.0 mg/mL of colchicines while that of R. mariesii increased 21.96% with 2.0 mg/mL. Stomata of three tested Rhododendrons varied with increase of colchicines concentration topped at 3.0 mg/mL. The variation of stomata density had no evident relation with polyploid. |
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| Keywords: | Colchicines Rhododendron simsii R ovatum R mariesii |
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